A kinetic study of the in vitro metabolism of 4-14C-dehydro-epiandrosterone by rat testis tissue
1966; Elsevier BV; Volume: 8; Issue: 4 Linguagem: Inglês
10.1016/0039-128x(66)90046-8
ISSN1878-5867
Autores Tópico(s)Pharmacological Effects and Assays
ResumoThe metabolism of 4-14C- dehydroepiandrosterone by rat testis tissues has been studied in vitro for four hours in several experiments, andresta-4, 16-dion-3-one being formed in every case. In addition, a more detailed kinetic study has been performed for periods of one, two, three and four hours. Steroid extracts have been separated by column and this layer chromatography coupled with autoradiography and the radioactivity of the following has been estimated: dehydroepi-androsterone, andrest-4-one-3,17-dione, androsterone, 5α-androstane-3α, 17β-diol, 5α-androstane-3β,17α-diol, androsta-4,16-dione- 3-one and, at four hours only, testosterone. Changes in these activities with time have been followed in an attempt to throw light on the biosynthetic pathway of the Δ16-keotone from DHA. The activity of this ketone, although low throughout, increased markedly up to two hours and thereafter remained constant. Antoradiographic evidence suggested that one or more androst-16-on-13-ols may also have been formed but the very small levels of radioactivity in these compounds did not allow farther purification. The radioactivity of 5α-androstane-3α, 17β-diol increased markedly and that of androstenodione decreased markedly as the time of incubation increased. The activities of the other metabolites measured remained relatively constant after the first 60 minutes of the experiment except for that of androsterone which decreased until the second hour, and then remained constant. The activity associatet with an unidentified compound less polar than the Δ4,16-ketone increased progressively with time.
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