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Interbase FRET in RNA: from A to Z

2019; Oxford University Press; Volume: 47; Issue: 19 Linguagem: Inglês

10.1093/nar/gkz812

1362-4962

Anders Foller Füchtbauer, Moa S. Wranne, Mattias Bood, Erik Weis, Pauline Pfeiffer, Jesper R. Nilsson, Anders Dahlén, Morten Grøtli, L. Marcus Wilhelmsson,

Protein Structure and Dynamics

Abstract Interbase FRET can reveal highly detailed information about distance, orientation and dynamics in nucleic acids, complementing the existing structure and dynamics techniques. We here report the first RNA base analogue FRET pair, consisting of the donor tCO and the non-emissive acceptor tCnitro. The acceptor ribonucleoside is here synthesised and incorporated into RNA for the first time. This FRET pair accurately reports the average structure of A-form RNA, and its utility for probing RNA structural changes is demonstrated by monitoring the transition from A- to Z-form RNA. Finally, the measured FRET data were compared with theoretical FRET patterns obtained from two previously reported Z-RNA PDB structures, to shed new light on this elusive RNA conformation.