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EFFECT OF EXHAUSTIVE ULTRA-ENDURANCE VS MODERATE EXERCISE ON RODENT CEREBELLUM ANTIOXIDANT CAPACITY

2019; Frontiers Media; Volume: 13; Linguagem: Inglês

10.3389/conf.fncel.2019.01.00018

1662-5102

Raphael Freitas de Souza, Ricielle Lopes Augusto, Sílvia Moraes, L. Buechner Vanessa, Danielle Dutra Pereira, Gisele Machado Magalhães Moreno, Fabio Souza, Fernanda Souza, Belmira Andrade- Da- Costa,

Genetics and Physical Performance

Event Abstract Back to Event Effect of exhaustive ultra-endurance vs moderate exercise on rodent cerebellum antioxidant capacity Raphael F. De Souza1*, Ricielle L. Augusto1, Silvia R. Moraes1, Lillian Vanessa1, Danielle D. Pereira1, Gisele M. Moreno1, Fabio B. Souza1, Fernanda M. Souza2 and Belmira L. Andrade- Da- Costa1 1 Federal University of Pernambuco, Brazil 2 Federal University of Alagoas, Brazil Ultra-endurance (UE) running increases O2 consumption and reactive oxygen species production in peripherical systems but it is still unknown how this type of training can affect brain regions involved in the movement control. The present study test the hypothesis that in addition to muscle damage, the cerebellum redox balance can be impaired by increasing training volumes. Forty five adult male Wistar rats were randomly subdivided in 6 groups according to the moderate training period followed or not by exhaustion test (ET) : control(C), Control + ET, moderate training volume (MV) moderate training volume + ET (MV-ET), high training volume (HV) and high training volume + ET (HV-ET). A continuous running characterized by low to moderate intensity was performed 5 times a week on a treadmill. The training period was gradually increased to 30 (MV) and 90 (HV) min/day over 12-week period. The velocity during ET, and the serum concentration of lactate (Lac), Creatine Kinase (CK) lactate dehydrogenase (LDH) and corticosterone were assessed. The cerebellum redox balance was analyzed by lipoperoxidation (LP) levels, reduced/ oxidized Glutathione GSH/GSSG ratio and the superoxide dismutase (SOD) and catalase (CAT) activities. Serum lactate levels were reduced in the MV-ET and HV-ET compared to CT-ET group (p<0.05) while CK levels were increased in the MV-ET, HV-ET and HV groups (p<0.03). Corticosterone concentration was higher in the HV and HV-ET contrast to MV-ET and C-ET groups (p≤0.05). Higher LP levels in the cerebellar tissue were induced by HV training despite the presence of ET. CAT activity was higher in the MV group compared to MV-ET, HV-ET and C groups (p<0.02). On the other hand, increased SOD activity was seen after ET independent of the running volume compared to C group (p≤0.05). Running velocity, GSH/GSSG ratio in the cerebellum, serum LDH and NO levels were not modified by the training. The data shows that the resilience of cerebellum to oxidative damage can be kept under moderate training volume but it is reduced by high volume in ultra-endurance running especially due to unbalanced antioxidant enzymatic activity. Keywords: Oxidative stress; central nervous system; exercise volume; catalase; superoxide dismutase; lipoperoxidation. Acknowledgements Coordination for the Improvement of Higher Level or Education Personnel (CAPES, Finance Code 001 and PROCAD-2013). Keywords: Oxidative stress ., Lipoperoxidation, superoxide dismutase ., Catalase, Central Nervous System Conference: XVI Meeting of the Portuguese Society for Neuroscience (SPN2019), Lisboa, Portugal, 30 May - 1 Jun, 2019. Presentation Type: Poster presentation Topic: Cellular and Molecular Neurosciences Citation: De Souza RF, Augusto RL, Moraes SR, Vanessa L, Pereira DD, Moreno GM, Souza FB, Souza FM and Andrade- Da- Costa BL (2019). Effect of exhaustive ultra-endurance vs moderate exercise on rodent cerebellum antioxidant capacity. Front. Cell. Neurosci. Conference Abstract: XVI Meeting of the Portuguese Society for Neuroscience (SPN2019). doi: 10.3389/conf.fncel.2019.01.00018 Copyright: The abstracts in this collection have not been subject to any Frontiers peer review or checks, and are not endorsed by Frontiers. They are made available through the Frontiers publishing platform as a service to conference organizers and presenters. The copyright in the individual abstracts is owned by the author of each abstract or his/her employer unless otherwise stated. Each abstract, as well as the collection of abstracts, are published under a Creative Commons CC-BY 4.0 (attribution) licence (https://creativecommons.org/licenses/by/4.0/) and may thus be reproduced, translated, adapted and be the subject of derivative works provided the authors and Frontiers are attributed. For Frontiers’ terms and conditions please see https://www.frontiersin.org/legal/terms-and-conditions. Received: 26 Feb 2019; Published Online: 27 Sep 2019. * Correspondence: Prof. Raphael F De Souza, Federal University of Pernambuco, Recife, Brazil, raphaelctba20@hotmail.com Login Required This action requires you to be registered with Frontiers and logged in. To register or login click here. Abstract Info Abstract The Authors in Frontiers Raphael F De Souza Ricielle L Augusto Silvia R Moraes Lillian Vanessa Danielle D Pereira Gisele M Moreno Fabio B Souza Fernanda M Souza Belmira L Andrade- Da- Costa Google Raphael F De Souza Ricielle L Augusto Silvia R Moraes Lillian Vanessa Danielle D Pereira Gisele M Moreno Fabio B Souza Fernanda M Souza Belmira L Andrade- Da- Costa Google Scholar Raphael F De Souza Ricielle L Augusto Silvia R Moraes Lillian Vanessa Danielle D Pereira Gisele M Moreno Fabio B Souza Fernanda M Souza Belmira L Andrade- Da- Costa PubMed Raphael F De Souza Ricielle L Augusto Silvia R Moraes Lillian Vanessa Danielle D Pereira Gisele M Moreno Fabio B Souza Fernanda M Souza Belmira L Andrade- Da- Costa Related Article in Frontiers Google Scholar PubMed Abstract Close Back to top Javascript is disabled. Please enable Javascript in your browser settings in order to see all the content on this page.