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High-Throughput Automated Microscopy of Circulating Tumor Cells

2019; Nature Portfolio; Volume: 9; Issue: 1 Linguagem: Inglês

10.1038/s41598-019-50241-w

2045-2322

Carlos Aguilar-Avelar, Brenda Soto‐García, Diana Araíz-Hernández, Juan Felipe Yee-de León, Miguel Esparza, Franco Chacón, Jesús Rolando Delgado-Balderas, Mario Moisés Álvarez, Grissel Trujillo‐de Santiago, Lauro Salvador Gómez-Guerra, Liza P. Velarde-Calvillo, Alejandro Abarca-Blanco, J. D. Wong-Campos,

3D Printing in Biomedical Research

Circulating tumor cells (CTCs) have the potential of becoming the gold standard marker for cancer diagnosis, prognosis and monitoring. However, current methods for its isolation and characterization suffer from equipment variability and human operator error that hinder its widespread use. Here we report the design and construction of a fully automated high-throughput fluorescence microscope that enables the imaging and classification of cancer cells that were labeled by immunostaining procedures. An excellent agreement between our machine vision-based approach and a state-of-the-art microscopy equipment was achieved. Our integral approach provides a path for operator-free and robust analysis of cancer cells as a standard clinical practice.