Portal de Periódicos da CAPES

First Report of Fruit Stain Caused by Moesziomyces aphidis on Grape in China

2019; American Phytopathological Society; Volume: 104; Issue: 2 Linguagem: Inglês

10.1094/pdis-07-19-1348-pdn

1943-7692

Su Ding, Mengji Cao, G. Chen, Shuifen Xie, QIAO-LAN HUANG, Jianwei Zhang, Guo Cheng, Shuangyun Zhou, Wei Li,

Yeasts and Rust Fungi Studies

HomePlant DiseaseVol. 104, No. 2First Report of Fruit Stain Caused by Moesziomyces aphidis on Grape in China PreviousNext DISEASE NOTES OPENOpen Access licenseFirst Report of Fruit Stain Caused by Moesziomyces aphidis on Grape in ChinaS. Ding, M. Cao, G. Chen, S. Xie, Q. Huang, J. Zhang, G. Cheng, S. Zhou, and W. LiS. DingCollege of Life Science and Technology, Guangxi University, Nanning 530004, Guangxi, China, M. CaoViticulture and Wine Research Institute, Guangxi Academy of Agricultural Sciences, Nanning 530007, Guangxi, China, G. ChenViticulture and Wine Research Institute, Guangxi Academy of Agricultural Sciences, Nanning 530007, Guangxi, China, S. XieViticulture and Wine Research Institute, Guangxi Academy of Agricultural Sciences, Nanning 530007, Guangxi, China, Q. HuangViticulture and Wine Research Institute, Guangxi Academy of Agricultural Sciences, Nanning 530007, Guangxi, China, J. ZhangViticulture and Wine Research Institute, Guangxi Academy of Agricultural Sciences, Nanning 530007, Guangxi, China, G. ChengViticulture and Wine Research Institute, Guangxi Academy of Agricultural Sciences, Nanning 530007, Guangxi, China, S. ZhouViticulture and Wine Research Institute, Guangxi Academy of Agricultural Sciences, Nanning 530007, Guangxi, China, and W. Li†Corresponding author: W. Li; E-mail Address: liweijt@qq.comhttp://orcid.org/0000-0003-3371-6008Viticulture and Wine Research Institute, Guangxi Academy of Agricultural Sciences, Nanning 530007, Guangxi, China AffiliationsAuthors and Affiliations S. Ding1 M. Cao2 G. Chen2 S. Xie2 Q. Huang2 J. Zhang2 G. Cheng2 S. Zhou2 W. Li2 † 1College of Life Science and Technology, Guangxi University, Nanning 530004, Guangxi, China 2Viticulture and Wine Research Institute, Guangxi Academy of Agricultural Sciences, Nanning 530007, Guangxi, China Published Online:30 Nov 2019https://doi.org/10.1094/PDIS-07-19-1348-PDNAboutSectionsSupplemental ToolsAdd to favoritesDownload CitationsTrack Citations ShareShare onFacebookTwitterLinked InRedditEmailWechat With the development of new technologies such as rain-shelter cultivation to improve growing conditions, table grape (Vitis vinifera) is now extensively cultivated in Guangxi province in southwestern China. In December 2011, whitish dry snowflake-like fruit stains on the surface of berries were first observed with an incidence over 90% on mature fruits of 'Kyoho' grape in a rain-shelter-cultivated vineyard (about 30 ha) near Nanning in south-central Guangxi province (N22.60, E108.23, 113 m altitude). This area is characterized by hot summers, mild winters, and an annual rainfall of at least 1,400 mm. In the following 8 years, this disease spread to many vineyards throughout Guangxi. Interestingly, the disease has led to a substantial decline in commodity value of grape berries but is not associated with decay. To identify the causal agent, stained tissues were scraped off and placed in a sterile 50-ml flask with glass beads and 10 ml of 0.9% saline solution containing 0.01% Tween-80 and resuspended by orbital shaking at 150 rpm for 1 h. The cell suspensions were diluted and spread on potato dextrose agar (PDA) plates. After 7 days in the dark at 25°C, single conidia were isolated from whitish yeast-like colonies and transferred to a yeast extract–malt extract agar plate. The pathogenicity of these isolates was tested by aerosol diffusion inoculation of spore suspensions on 30 bunches of Kyoho grape that were surface sterilized using sodium hypochlorite. After 14 days, typical symptoms occurred of fruit stains, whereas control fruits (inoculated with sterile water only) remained symptomless. The reisolation of the pathogens from symptomatic inoculated fruits on PDA after 7 days at 25°C allowed the purification of strains with the same morphological features as the original isolates, which fulfilled Koch's postulates. Among 25 yeast-like original isolates, 17 isolates showed pathogenicity of fruit stain. Among them, two strains named GX-1602 and GX-1701 with relative higher pathogenicity were picked out for further investigation. Microscopic observation of approximately 50 blastoconidia with more or less fusiform shape found that their conidia were 3.5 to 9.0 μm long and 1.7 to 3.0 μm wide. Total DNA of the isolates was extracted with a Universal Genomic DNA Extraction Kit (TaKaRa, Japan), and morphological criteria were confirmed by sequencing the ITS region of rDNA amplified with primers pair ITS1 and ITS4. The resulting sequences deposited in GenBank (MK994025 and MK994026) showed 99.62% homology (778 base pairs match) with Moesziomyces aphidis JCM 10318 sequence type (formerly Pseudozyma aphidis). A phylogenetic analysis performed with the neighbor-joining method based on the maximum composite likelihood model (1,000 bootstrap replications) confirmed the identity of the isolates to species M. aphidis. This anamorph yeast-like basidiomycete was originally reported to be a saprotroph of flowers (Wei et al. 2005), an endophytic yeast in pineapple fruit (Tristão et al. 2012), and a human pathogen causing opportunistic disease (de Carvalho Parahym et al. 2013). To our best knowledge, although M. aphidis is a fruit pathogen on pear in Japan (Yasuda et al. 2007), this is the first report on M. aphidis causing fruit stain on grape in China. Previous research from our lab showed that M. aphidis is one of the most abundant epiphytic fungal species on the surface of Kyoho grape berries during the ripening process in this region (Ding et al. 2019), indicating that M. aphidis should be considered as an emerging threat for grape production in Guangxi, China.The author(s) declare no conflict of interest.References:de Carvalho Parahym, A. M. R., et al. 2013. Diagn. Microbiol. Infect. Dis. 75:104. https://doi.org/10.1016/j.diagmicrobio.2012.09.010 Crossref, ISI, Google ScholarDing, S., et al. 2019. Fungal Biol. 123:283. https://doi.org/10.1016/j.funbio.2018.11.014 Crossref, ISI, Google ScholarTristão, G. B., et al. 2012. Eng. Ambiental – Espírito Santo do Pinhal 9:85. Google ScholarWei, Y. H., et al. 2005. Bot. Bull. Acad. Sin. 46:223. Google ScholarYasuda, F., et al. 2007. Jap. J. Phytopathol. 73:166. https://doi.org/10.3186/jjphytopath.73.166 Crossref, Google ScholarS. Ding and M. Cao contributed equally to this work.The author(s) declare no conflict of interest.Funding: This study was financially supported by Guangxi Key Technologies R&D Program (grant no. AB18221037), the Guangxi Natural Science Foundation (grant no. 2018GXNSFAA138062), and the Sciences and Technology Development Fund of Guangxi Academy of Agricultural Sciences (grant no. 2018YT32).DetailsFiguresLiterature CitedRelated Vol. 104, No. 2 February 2020SubscribeISSN:0191-2917e-ISSN:1943-7692 DownloadCaptionSymptom of maize ear rot caused by Fusarium sporotrichioides (B. B. Wang et al.). Photo credit: C. X. Duan. Systemic symptoms of alfalfa mosaic virus (AMV) isolate CaM on leaves of potato (X. Z. Nie et al.). Photo credit: X. Z. Nie. Metrics Downloaded 1,451 times Article History Issue Date: 31 Jan 2020Published: 30 Nov 2019First Look: 23 Sep 2019Accepted: 16 Sep 2019 Page: 586 Information© 2020 The American Phytopathological SocietyFundingGuangxi Key Technologies R&D ProgramGrant/Award Number: AB18221037Guangxi Natural Science FoundationGrant/Award Number: 2018GXNSFAA138062Sciences and Technology Development Fund of Guangxi Academy of Agricultural SciencesGrant/Award Number: 2018YT32KeywordsMoesziomyces aphidisfruit staingrapeThe author(s) declare no conflict of interest.