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A Novel Amperometric Sensor for Organophosphotriester Insecticides Detection Employing Catalytic Polymer Mimicking Phosphotriesterase Catalytic Center

2001; The Electrochemical Society of Japan; Volume: 69; Issue: 12 Linguagem: Inglês

10.5796/electrochemistry.69.969

2186-2451

Tomohiko Yamazaki, Zihui Meng, Klaus Mosbach, Koji Sode,

Electrochemical Analysis and Applications

An amperometric sensor for organophosphotriester insecticides employing polymer catalysis, as an artificial enzyme, was developed. The polymer catalysis was composed of the Co2+-imidazoles complexes which is the mimic of the catalytic center of the organophosphotriester insecticides hydrolysis enzyme, phosphotriesterase (PTE). The polymer catalysis enabled to hydrolyze paraoxon to p-nitrophenol and diethylphosphoric acid. The hydrolysate p-nitrophenol was subsequently oxidized on the anodic electrode, at the sensor system employing polymer catalysis. The lower detection limit was 100 μM for paraoxon at 40°C in 4 min.