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Lipid production in Rhodosporidium toruloides using C-6 and C-5 wood hydrolysate: A comparative study

2019; Elsevier BV; Volume: 130; Linguagem: Inglês

10.1016/j.biombioe.2019.105355

1873-2909

Carlos S. Osorio‐González, Krishnamoorthy Hegde, Pedro Ferreira, Satinder Kaur Brar, Azadeh Kermanshahi‐pour, Carlos Ricardo Soccol, Antonio Avalos Ramírez,

Enzyme Catalysis and Immobilization

Rhodosporidium toruloides is an oleaginous yeast that can accumulate up to 70% of its dry biomass as lipids and can use C5 and C6 sugars as a carbon source, that are present in hydrolysates from different lignocellulosic biomass. R. toruloides can be an alternative for the valorization of forestry residues and the obtention of higher value-added products. In this study, five strains of R. toruloides were evaluated to find the most efficient yeast strain for C5 and C6 sugars utilization, using lignocellulosic hydrolysates as a culture media. Sugar consumption was similar between all strains. Nevertheless, it was different among C6 and C5 hydrolysates, with maximum sugar utilization of 98%, and 60% for C6 and C5 hydrolysate, respectively. Among the studied strains, the highest lipid production was observed in R. toruloides-1588 with 23.33 g of lipids/L in C6 hydrolysate after 112 h. Whereas, highest lipid production in C5 hydrolysate was observed in R. toruloides-7191 with 14.67 g of lipids/L after 120 h. Predominantly, fatty acids for R. toruloides-1588 was oleic, palmitic, and stearic acids and pentadecanoic, palmitic, and heptadecanoic acids for R. toruloides-7191. Moreover, both strains grew and produced lipids in the presence of inhibitor compounds, such as levulinic acid (13.88 mgL−1), 5-HMF (80.86 mgL−1), furfural (153.54 mgL−1), vanillin (17.17 mgL−1), vanillic acid (85.25 mgL−1), syringaldehyde (41.83 mgL−1), ferulic acid (1.66 mgL−1), and 4-hydroxybenzoic acid (0.85 mgL−1). The study revealed that R. toruloides-1588 and R. toruloides-7191 are promising strains for C5 and C6 sugar utilization from lignocellulosic hydrolysates for lipid production.