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Beneficial effect of geniposide on promoting implant osseointegration in T2DM rats

2019; Wiley; Volume: 30; Issue: S19 Linguagem: Inglês

10.1111/clr.57_13509

1600-0501

Tingting Jia,

Neurological Disorders and Treatments

Background Accumulating evidence has shown that diabetes-induced impaired osseointegration of titanium implant would result high failure rate of implantation. Geniposide, the main components of gardenia, plays an important role in glucose metabolism, inflammation control and bone regeneration. Aim/Hypothesis Considering the osseointegration follows the principles of metabolic regulation and bone regeneration, we have raised the hypothesis that geniposide could reverse compromised osseointegration in type 2 diabetes mellitus (T2DM) rats. Materials and Methods 1. Inducement of T2DM model: After adaptive breeding, model rats were fed with high-fat diet for 1 month to induce T2DM, then were injected with streptozotocin solution (STZ, Sigma) at a dose of 30 mg/kg. Fasting blood glucose (FBG) ≥ 13.9 mmol/L was considered to be hyperglycemia for further research. 2. Surgery and Treatment: Model Rats were randomly divided into T2DM group and geniposide-treated T2DM group, then normal rats as control group. Ti implant (φ1 mm *10 mm) were implanted into intercondylar fossa of rats after anesthesia. Three days later, the treatment group received geniposide every day. FBG and body weight were measured every 2 weeks after treatment. 3. Correlation detections: All animals were euthanatized at the 12th week and the specimens were used to undertake continue experiment, such as pull-out test, microscopic computerized tomography (micro-CT) analysis, and histological observation. Results 1. FBG levels and body weight: The successful T2DM rat models were correlated with FBG increase and weight loss, which were obviously higher and lower than controls (P < 0.05). The administration of geniposide could ameliorate them in T2DM rats (P < 0.05).2. Pull-out test: Significant increases were observed in the values of the biomechanical index in geniposide treatment group, compared to T2DM model rats (P < 0.05). 3. Histological observation: Histological observation showed geniposide treatment increased peri-implant bone mass in diabetic rats. And the index of BIC in model rats was significantly less than control and geniposide treatment group(P < 0.05).4. Micro-CT analysis: Diabetic model displayed the more bone loss, the less and thinner bone trabecula, and geniposide treatment reversed these injuries significantly. The BV TV, %OI and Tb.N in the T2DM group lessened more dramatically than the control and geniposide-treated group(P < 0.05). Conclusion and Clinical Implications The study revealed that geniposide could promote implant osseointegration under hyperglycemia condition, and provided a theoretical basis that geniposide could work as an adjunct treatment to against poor osseointegration in diabetic patients.