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First Report of the Fusarium tricinctum Species Complex Causing Fusarium Head Blight of Wheat in Brazil
2019; American Phytopathological Society; Volume: 104; Issue: 2 Linguagem: Inglês
10.1094/pdis-03-19-0552-pdn
ISSN1943-7692
AutoresGláucia M. Moreira, Franklin Jackson Machado, Carolina Bertuzzi Pereira, D. L. Neves, Dauri José Tessmann, Todd J. Ward, Emerson M. Del Ponte,
Tópico(s)Plant Disease Resistance and Genetics
ResumoHomePlant DiseaseVol. 104, No. 2First Report of the Fusarium tricinctum Species Complex Causing Fusarium Head Blight of Wheat in Brazil PreviousNext DISEASE NOTES OPENOpen Access licenseFirst Report of the Fusarium tricinctum Species Complex Causing Fusarium Head Blight of Wheat in BrazilG. M. Moreira, F. J. Machado, C. B. Pereira, D. L. Neves, D. J. Tessmann, T. J. Ward, and E. M. Del PonteG. M. MoreiraDepartamento de Fitopatologia, Universidade Federal de Viçosa, Viçosa MG, 36570-900, Brazil, F. J. MachadoDepartamento de Fitopatologia, Universidade Federal de Viçosa, Viçosa MG, 36570-900, Brazil, C. B. PereiraDepartamento de Agronomia, Universidade Estadual de Maringá, Maringá PR, 87020-900, Brazil, D. L. NevesDepartamento de Agronomia, Universidade Estadual de Maringá, Maringá PR, 87020-900, Brazil, D. J. TessmannDepartamento de Agronomia, Universidade Estadual de Maringá, Maringá PR, 87020-900, Brazil, T. J. WardUSDA-ARS, Peoria, IL 61604-3999, U.S.A., and E. M. Del Ponte†Corresponding author: E. M. Del Ponte; E-mail Address: delponte@ufv.brhttp://orcid.org/0000-0003-4398-409XDepartamento de Fitopatologia, Universidade Federal de Viçosa, Viçosa MG, 36570-900, Brazil AffiliationsAuthors and Affiliations G. M. Moreira1 F. J. Machado1 C. B. Pereira2 D. L. Neves2 D. J. Tessmann2 T. J. Ward3 E. M. Del Ponte1 † 1Departamento de Fitopatologia, Universidade Federal de Viçosa, Viçosa MG, 36570-900, Brazil 2Departamento de Agronomia, Universidade Estadual de Maringá, Maringá PR, 87020-900, Brazil 3USDA-ARS, Peoria, IL 61604-3999, U.S.A. Published Online:30 Nov 2019https://doi.org/10.1094/PDIS-03-19-0552-PDNAboutSectionsSupplemental ToolsAdd to favoritesDownload CitationsTrack Citations ShareShare onFacebookTwitterLinked InRedditEmailWechat Fusarium graminearum species complex (FGSC) is the main cause of Fusarium head blight (FHB) in Brazil. However, during surveys conducted from 2008 to 2011, 26 wheat kernel-borne or airborne (air above wheat canopy) strains failed using a FGSC-specific multilocus genotyping assay (Del Ponte et al. 2015). Eight spike-borne strains originating from south of Paraná (PR) state (two locations), 11 airborne strains from one field at Passo Fundo (RS), and seven kernel-borne strains each from a different location in northern RS state were subjected to amplification and sequencing of the translation elongation factor 1-alpha gene (primers EF1/EF2, O'Donnell et al. 1998). Maximum parsimony- and maximum likelihood-based methods were fitted using MEGA X (Kumar et al. 2018). Eleven strains were also sequenced for the second largest subunit of RNA polymerase II (primers 5f2/7cr, O'Donnell et al. 2008). The genetic analyses used either the single- or the two-gene datasets. Sequences were deposited in GenBank (MK572748 to MK572784). Three species of the Fusarium tricinctum species complex (FTSC) were identified: 15 F. avenaceum, nine FTSC 11, and two F. reticulatum. Although the two most frequent species were either airborne or kernel-borne, F. reticulatum was only airborne. Among the strains from PR, FTSC 11 was represented by one strain and the rest by F. avenaceum. In RS, FTSC 11 and F. avenaceum, airborne or kernel-borne, were in similar frequency. Eighteen strains representative of all species were subjected to pathogenicity assays on an FHB-susceptible spring wheat (BR18). Two standard FGSC strains, F. graminearum (CML 3066) and F. meridionale (CML 3344), were included for comparison. Three potted wheat plants (replicates) were used. Fungi were grown on Spezieller Nährstoffarmer agar for 7 days at 25°C with a 12-h photoperiod. A macroconidia suspension (1 × 104 macroconidia/ml) was applied uniformly onto wheat heads at flowering using a household handheld sprayer (2 ml/head). Plants were covered with a plastic bag for 48 h and remained in the greenhouse until harvest (35 days). Disease severity was assessed visually at 4 and 8 days postinoculation (dpi) on each inoculated head. The assay was repeated once. All strains were pathogenic to wheat, and the reisolation of at least two strains from infected spikes confirmed their identity. FHB severity at 4 dpi ranged from 11.7 to 82.8% for F. avenaceum, from 14.1 to 46.2% for F. reticulatum, and from 14.1 to 90.2% for FTSC 11. Similarly, a large intraspecies variation of mean FHB severity was observed at 8 dpi for F. avenaceum (33 to 94.5%), F. reticulatum (32.6 to 79.6%), and FTSC 11 (42.2 to 97.8%). Although evidence for a species effect was not strong (P = 0.08), some strains of all species induced FHB at levels similar to FGSC. FTSC members are known to prevail in cooler climates such as Canada and northern Europe (Bottalico and Perrone 2002; Gräfenhan et al. 2013). They were reported in Kentucky (U.S.A.) wheat, including F. reticulatum strains that were weakly pathogenic toward wheat (Bec et al. 2015). In Brazil, barley from the 2016 growing season was found to harbor FTSC strains (Piacentini et al. 2019). Further work is needed to understand whether FTSC is an emergent FHB pathogen, especially because of their known ability to produce enniatins and beauvericin mycotoxins, which are not regulated in Brazil.The author(s) declare no conflict of interest.References:Bec, S., et al. 2015. Plant Dis. 99:1622. https://doi.org/10.1094/PDIS-06-14-0610-RE Link, ISI, Google ScholarBottalico, A., and Perrone, G. 2002. Eur. J. Plant Pathol. 108:611. https://doi.org/10.1023/A:1020635214971 Crossref, ISI, Google ScholarDel Ponte, E. M., et al. 2015. Phytopathol. 105:246. https://doi.org/10.1094/PHYTO-04-14-0102-R Link, ISI, Google ScholarGräfenhan, T., et al. 2013. J. Agric. Food Chem. 61:5425. https://doi.org/10.1021/jf400651p Crossref, ISI, Google ScholarKumar, S., et al. 2018. Mol. Biol. Evol. 35:1547. https://doi.org/10.1093/molbev/msy096 Crossref, ISI, Google ScholarO'Donnell, K., et al. 1998. Proc. Natl. Acad. Sci. U.S.A. 95:2044. https://doi.org/10.1073/pnas.95.5.2044 Crossref, ISI, Google ScholarO'Donnell, K., et al. 2008. J. Clin. Microbiol. 46:2477. https://doi.org/10.1128/JCM.02371-07 Crossref, ISI, Google ScholarPiacentini, K. C., et al. 2019. Toxins (Basel) 11:31. https://doi.org/10.3390/toxins11010031 Crossref, Google ScholarThe author(s) declare no conflict of interest.DetailsFiguresLiterature CitedRelated Vol. 104, No. 2 February 2020SubscribeISSN:0191-2917e-ISSN:1943-7692 DownloadCaptionSymptom of maize ear rot caused by Fusarium sporotrichioides (B. B. Wang et al.). Photo credit: C. X. Duan. Systemic symptoms of alfalfa mosaic virus (AMV) isolate CaM on leaves of potato (X. Z. Nie et al.). Photo credit: X. Z. Nie. Metrics Downloaded 2,394 times Article History Issue Date: 31 Jan 2020Published: 30 Nov 2019First Look: 23 Sep 2019Accepted: 16 Sep 2019 Pages: 586-586 Information© 2020 The American Phytopathological SocietyKeywordswheat scabTriticum aestivummycotoxigenic fungiThe author(s) declare no conflict of interest.Cited ByFungal community composition and diversity in the rhizosphere soils of Argentina (syn. 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