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First Report of Fusarium Species Associated with Fusarium Wilt in Coffea canephora Plants in Brazil

2018; American Phytopathological Society; Volume: 102; Issue: 9 Linguagem: Inglês

10.1094/pdis-01-18-0186-pdn

1943-7692

Leônidas Leoni Belan, Leonardo Leoni Belan, Arango Rafael, Rodrigo Monte Lorenzoni, F. B. Souza-Sobreira, Taís Cristina Bastos Soares, Fábio Luíz de Oliveira, Willian Bucker Moraes,

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HomePlant DiseaseVol. 102, No. 9First Report of Fusarium Species Associated with Fusarium Wilt in Coffea canephora Plants in Brazil PreviousNext DISEASE NOTES OPENOpen Access licenseFirst Report of Fusarium Species Associated with Fusarium Wilt in Coffea canephora Plants in BrazilL. L. Belan, L. L. Belan, A. M. Rafael, R. M. Lorenzoni, F. B. Souza-Sobreira, T. C. B. Soares, F. L. de Oliveira, and W. B. MoraesL. L. Belanhttp://orcid.org/0000-0002-7966-4963, L. L. Belanhttp://orcid.org/0000-0002-3741-4173, A. M. Rafael, R. M. Lorenzoni, F. B. Souza-Sobreira, T. C. B. Soares, F. L. de Oliveira, and W. B. Moraes†Corresponding author: W. B. Moraes; E-mail: E-mail Address: willian.moraes@ufes.brhttp://orcid.org/0000-0001-7478-7772AffiliationsAuthors and Affiliations L. L. Belan L. L. Belan A. M. Rafael , Department of Agronomy, CCAE-UFES, 29500-000 Alegre-ES, Brazil R. M. Lorenzoni F. B. Souza-Sobreira , Graduate Program in Vegetal Production, CCAE-UFES, 29500-000 Alegre-ES, Brazil T. C. B. Soares , Department of Pharmacy and Nutrition, UFES, 29500-000 Alegre-ES, Brazil F. L. de Oliveira W. B. Moraes † , Department of Agronomy, CCAE-UFES, 29500-000 Alegre-ES, Brazil. Published Online:24 Jul 2018https://doi.org/10.1094/PDIS-01-18-0186-PDNAboutSectionsSupplemental ToolsAdd to favoritesDownload CitationsTrack Citations ShareShare onFacebookTwitterLinked InRedditEmailWechat Coffea canephora plants have been wilting and dying in major producing regions of Brazil; however, the disease etiology is unknown. Coffee clones differ in susceptibility, and disease occurs throughout the year. The symptoms include wilting, leaf yellowing, defoliation, vascular tissue darkening, and drying and death of plagiotropic and orthotropic branches and the plant. To identify the etiological agent, vascular tissue fragments from symptomatic plants collected in the Jerônimo Monteiro municipality (20°53′28″S, 41°25′59″W) were surface sterilized, placed on carrot baits, and incubated at 26°C. The resulting mycelia were transferred to potato dextrose agar (PDA) medium and incubated at 26°C in 12-h light/dark for 10 days. Monosporic cultures from four isolates were prepared and characterized (Booth 1971; Summerell et al. 2003). A TEF-1α gene fragment was amplified (O'Donnell et al. 2000). The polymerase chain reaction products were sequenced by capillary electrophoresis (ABI3730) using POP7 polymer and BigDye version 3.1 (Myleus Biotechnology). We identified three species of Fusarium using BLAST analysis: F. decemcellulare and F. lateritium with 100% sequence similarity to GenBank accessions numbers KC491872.1 and AY707155.1, respectively, and F. solani with 99% similarity (DQ247549.1). F. decemcellulare colonies were cottony and pinkish to yellowish, with yellow or cream globular sporodochia; macroconidia (70.4 to 99.3 × 7.2 to 8.1 μm) were fusiform, with a beaked apical cell, a distinct pedicellate foot cell and 6 to 9 septa; microconidia (7.3 to 12.5 × 3.6 to 6.1 μm) were hyaline, oval, with a flattened basal papilla and 0 to 1 septum. F. lateritium colonies showed slow growth, with white to peach aerial mycelium; macroconidia (33.5 to 46.0 × 3.9 to 5.4 μm) with a beaked apical cell and 3 to 5 septa; microconidia (8.6 to 17.4 × 2.7 to 4.3 μm) were fusiform with 0 to 1 septum. F. solani colonies showed rapid growth and white to cream colored aerial mycelium with flat and globular cream-colored sporodochia; macroconidia (29.5 to 50.3 × 5.0 to 8.1 μm) were hyaline with 3 septa; microconidia (9.6 to 14.9 × 4.0 to 6.3 μm) were hyaline, unicellular, and oblong, with 0 to 2 septa. Coffee plants (4 months old) of clone CV02 and plantlets (40 days old) of Robusta Tropical cultivar were inoculated. The inoculation was realized with a monosporic isolate of each species. Ten coffee plants were inoculated with PDA disks containing 10-day-old colony, or sterile PDA disk (control), inserted into a stem puncture. The plants were maintained in a humid chamber for 48 h (100% relative humidity). The roots of 10 coffee plantlets were immersed for 30 min in a conidia suspension (106 conidia/ml) or in sterile water (control) prior to transplantation. The first symptoms of impaired growth, vascular tissue darkening, leaf chlorosis, wilting, and defoliation appeared 45 days after inoculation of coffee plants. Inoculated plantlets wilted and died after 65 days. Control plants and plantlets did not develop symptoms. The fungi reisolated from diseased plants were similar to those isolated from the original host. Another Fusarium sp. has been reported with this host in Puerto Rico, Virgin Islands, Fiji, and Cote d'Ivoire, but to our knowledge, this is the first report of F. decemcellulare, F. lateritium, and F. solani as etiological agents of Fusarium wilt in C. canephora. Studies on this pathosystem are necessary considering that the size of the area affected and losses are increasing, and 100% damage can occur in crops of susceptible cultivars.References:Booth, C., ed. 1971. The Genus Fusarium. CABI, Kew, England. Google ScholarO'Donnell, K., et al. 2000. Mycoscience 41:61. https://doi.org/10.1007/BF02464387 Crossref, Google ScholarSummerell, B. A., et al. 2003. Plant Dis. 87:117. https://doi.org/10.1094/PDIS.2003.87.2.117 Link, ISI, Google ScholarDetailsFiguresLiterature CitedRelated Vol. 102, No. 9 September 2018SubscribeISSN:0191-2917e-ISSN:1943-7692 Metrics Article History Issue Date: 20 Aug 2018Published: 24 Jul 2018First Look: 17 Apr 2018Accepted: 14 Apr 2018 Pages: 1859-1859 Information© 2018 The American Phytopathological SocietyCited byCharacterization of Fusarium spp. causal agent of wilt in pecan1 November 2022 | Revista Ceres, Vol. 69, No. 6Albonectria rigidiuscula (green point gall)CABI Compendium, Vol. CABI CompendiumHaematonectria haematococca (dry rot of potato)CABI Compendium, Vol. CABI CompendiumGibberella baccata (collar rot of coffee)CABI Compendium, Vol. CABI CompendiumDigitodesmium polybrachiatum sp. nov., a new species of Dictyosporiaceae from Brazil2 October 2021 | Mycological Progress, Vol. 20, No. 9First Report of Fusarium Wilt Caused by Fusarium oxysporum on Pecan in BrazilJ. M. Rolim, L. G. Savian, C. Walker, E. 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