First Report of Pantoea stewartii subsp. indologenes Causing Leaf Blight on Rice in Malaysia
2019; American Phytopathological Society; Volume: 103; Issue: 6 Linguagem: Inglês
10.1094/pdis-08-18-1403-pdn
ISSN1943-7692
AutoresMohammad Malek Faizal Azizi, Siti Izera Ismail, Erneeza Mohd Hata, Dzarifah Zulperi, Ina Salwany Md Yasin, M. A. F. Abdullah,
Tópico(s)Legume Nitrogen Fixing Symbiosis
ResumoHomePlant DiseaseVol. 103, No. 6First Report of Pantoea stewartii subsp. indologenes Causing Leaf Blight on Rice in Malaysia PreviousNext DISEASE NOTESFirst Report of Pantoea stewartii subsp. indologenes Causing Leaf Blight on Rice in MalaysiaM. M. F. Azizi, S. I. Ismail, E. M. Hata, D. Zulperi, M. Y. Ina-Salwany, and M. A. F. AbdullahM. M. F. AziziDepartment of Plant Protection, Faculty of Agriculture, Universiti Putra Malaysia, 43400 Serdang, Selangor, Malaysia, S. I. IsmailDepartment of Plant Protection, Faculty of Agriculture, Universiti Putra Malaysia, 43400 Serdang, Selangor, Malaysia, E. M. HataDepartment of Plant Protection, Faculty of Agriculture, Universiti Putra Malaysia, 43400 Serdang, Selangor, Malaysia, D. Zulperi†Corresponding author: D. Zulperi; E-mail Address: [email protected]http://orcid.org/0000-0002-3406-9730Department of Plant Protection, Faculty of Agriculture, Universiti Putra Malaysia, 43400 Serdang, Selangor, Malaysia, M. Y. Ina-SalwanyDepartment of Aquaculture, Faculty of Agriculture, Universiti Putra Malaysia, 43400 Serdang, Selangor, Malaysia, and M. A. F. AbdullahAgribolics Technology Sdn Bhd, UPM-MTDC Technology Centre, Universiti Putra Malaysia, 43400 Serdang, Selangor, MalaysiaAffiliationsAuthors and Affiliations M. M. F. Azizi1 S. I. Ismail1 E. M. Hata1 D. Zulperi1 † M. Y. Ina-Salwany2 M. A. F. Abdullah3 1Department of Plant Protection, Faculty of Agriculture, Universiti Putra Malaysia, 43400 Serdang, Selangor, Malaysia 2Department of Aquaculture, Faculty of Agriculture, Universiti Putra Malaysia, 43400 Serdang, Selangor, Malaysia 3Agribolics Technology Sdn Bhd, UPM-MTDC Technology Centre, Universiti Putra Malaysia, 43400 Serdang, Selangor, Malaysia Published Online:19 Apr 2019https://doi.org/10.1094/PDIS-08-18-1403-PDNAboutSections ToolsAdd to favoritesDownload CitationsTrack Citations ShareShare onFacebookTwitterLinked InRedditEmailWechat In November and December of 2017, leaf samples from rice (Oryza sativa) exhibiting leaf blight symptoms were collected from commercial fields in Kedah and Selangor, Malaysia, with about 80% disease incidence. Symptoms were water-soaked lesions from the upper part of the leaf blade, which later turned into brown stripes (Mondal et al. 2011), as typically caused by Xanthomonas oryzae pv. oryzae (Niño-Liu et al. 2006). Twenty strains of bacteria were isolated from the diseased samples from both fields, and eight isolates (MF1, MF2, MF3, MF4, MF5, MF7, MF8, and MF9) suspected to be Pantoea species were subjected to further characterization. Bacterial colonies were isolated from surface-sterilized infected leaf tissue using 75% ethanol and 1% sodium hypochlorite, rinsed three times in sterilized water, macerated in sterilized water, placed on King's B agar medium, and incubated for 48 h at 28°C. Single colonies were transferred and maintained on nutrient broth medium. All isolated bacteria were gram-negative facultative anaerobes, motile, positive for catalase and gelatin tests, hydrolyzing starch, incapable of producing hydrosulfuric acid, indole positive, and able to produce acetoin. Colonies were round, smooth with irregular edges, and produced yellow pigment on King's B agar medium. Polymerase chain reaction using specific primers targeting the gyrb gene of Pantoea genome was performed, and the ∼600-bp amplicons were sequenced (Kini et al. 2017). BLASTn analysis revealed all strains had 99% similarity to the Pantoea stewartii reference strain (ARC646) in the GenBank database (accession no. KX342016). These sequences were later deposited in GenBank (accession nos. MH300616 to MH300621 and MH673052 to MH673054). A phylogenetic tree constructed based on the concatenated nucleotide sequences of the gyrb gene showed that all strains were 99% similar to P. stewartii reference strains (accession nos. KX342015, KF554590, KT7295191, and KX342016). The strains were tested with subspecies-specific primers of P. stewartii subsp. stewartii and P. stewartii subsp. indologenes targeting housekeeping gene gaIE that encodes UDP-glucose 4-epimerase to confirm the subspecies (Gehring et al. 2014). Positive bands were produced by all strains from gaIE indologenes subspecies, each producing a ∼267-bp amplicon. All strains were identical to the reference strain of P. stewartii subsp. indologenes (NCPPB 1845) in GenBank database (accession no. HG792431). To test pathogenicity, 10 ml of 108 CFU/ml bacterial suspension of each strain was inoculated into 4-week-old rice seedlings of MR269 variety using the leaf clipping method (Kauffman et al. 1973), and they were kept in the greenhouse with temperature ranging from 26 to 35°C. All eight strains of P. stewartii subsp. indologenes produced symptoms similar to those originally found in the rice fields, including yellowish necrotic water-soaked lesions, which later turned to typical blight at 2 weeks postinoculation. Strains were reisolated from diseased leaves using the same method mentioned above and confirmed as P. stewartii subsp. indologenes by sequence analysis of the gaIE gene, thus fulfilling Koch's postulates. To the best of our knowledge, this is the first report of leaf blight disease caused by P. stewartii subsp. indologenes on rice in Malaysia.The author(s) declare no conflict of interest.References:Gehring, I., et al. 2014. J. Appl. Microbiol. 116:1553. https://doi.org/10.1111/jam.12467 Crossref, ISI, Google ScholarKauffman, H. E., et al. 1973. Plant Dis. Rep. 57:537. ISI, Google ScholarKini, K., et al. 2017. Plant Dis. 101:241. https://doi.org/10.1094/PDIS-06-16-0939-PDN Link, ISI, Google ScholarMondal, K. K., et al. 2011. Plant Dis. 95:1582. https://doi.org/10.1094/PDIS-06-11-0533 Link, ISI, Google ScholarNiño-Liu, D. O., et al. 2006. Mol. Plant Pathol. 7:303. https://doi.org/10.1111/j.1364-3703.2006.00344.x Crossref, ISI, Google ScholarThe author(s) declare no conflict of interest.Funding: Funding was provided by UPM-IPM (grant no. 9494600).DetailsFiguresLiterature CitedRelated Vol. 103, No. 6 June 2019SubscribeISSN:0191-2917e-ISSN:1943-7692 DownloadCaptionGreen mottle mosaic and leaf deformation symptoms on watermelon (Sui, Li, Shamimuzzaman, Wu, and Ling). Photo credit: K.-S. Ling. Postharvest rot on cucumber caused by Ceratocystis fimbriata (Li, Xu, Zhang, Song, Xie, Sun, and Huang). Photo credit: H. Song. Metrics Article History Issue Date: 6 Jun 2019Published: 19 Apr 2019First Look: 7 Mar 2019Accepted: 4 Mar 2019 Pages: 1407-1407 Information© 2019 The American Phytopathological SocietyFundingUPM-IPMGrant/Award Number: 9494600Keywordsprokaryotesfield cropscereals and grainspathogen detectionThe author(s) declare no conflict of interest.Cited byComplete Genome Sequence of Pantoea stewartii RON18713 from Brazil Nut Tree Phyllosphere Reveals Genes Involved in Plant Growth Promotion30 June 2023 | Microorganisms, Vol. 11, No. 7Analysis of Italian isolates of Pantoea stewartii subsp. stewartii and development of a real-time PCR-based diagnostic method27 April 2023 | Frontiers in Microbiology, Vol. 14Leaf blight disease of rice caused by Pantoea: profile of an increasingly damaging disease in rice16 November 2022 | Tropical Plant Pathology, Vol. 48, No. 1Beneficial Effect and Potential Risk of Pantoea on Rice Production4 October 2022 | Plants, Vol. 11, No. 19Advanced diagnostic approaches developed for the global menace of rice diseases: a review5 April 2022 | Canadian Journal of Plant Pathology, Vol. 44, No. 5Biology, Diagnostics, Pathogenomics and Mitigation Strategies of Jackfruit-Bronzing Bacterium Pantoea stewartii subspecies stewartii: What Do We Know So Far about This Culprit?3 August 2022 | Horticulturae, Vol. 8, No. 8Current and emerging molecular technologies for the diagnosis of plant diseases – An overview30 April 2022 | Journal of Experimental Biology and Agricultural Sciences, Vol. 10, No. 2Pantoea stewartii (bacterial wilt of maize)CABI Compendium, Vol. CABI CompendiumAssociations of Pantoea with Rice Plants: As Friends or Foes?16 December 2021 | Agriculture, Vol. 11, No. 12Identification of Two Novel Pathovars of Pantoea stewartii subsp. indologenes Affecting Allium sp. and MilletsSantosh Koirala, Mei Zhao, Gaurav Agarwal, Ron Gitaitis, Shaun Stice, Brian Kvitko, and Bhabesh Dutta14 October 2021 | Phytopathology®, Vol. 111, No. 9Plant Growth-Promoting Bacteria as an Emerging Tool to Manage Bacterial Rice Pathogens26 March 2021 | Microorganisms, Vol. 9, No. 4Pantoea: a newly identified causative agent for leaf blight disease in rice6 July 2019 | Journal of Plant Diseases and Protection, Vol. 126, No. 6
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