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Nanoluciferase-Based Method for Detecting Gene Expression in Caenorhabditis elegans

2019; Oxford University Press; Volume: 213; Issue: 4 Linguagem: Inglês

10.1534/genetics.119.302655

1943-2631

Ivana Sfarcic, Theresa Bui, Erin Daniels, Emily R. Troemel,

CRISPR and Genetic Engineering

Genetic reporters such as the green fluorescent protein (GFP) can facilitate measurement of promoter activity and gene expression. However, animal autofluorescence limits the sensitivity of GFP and other fluorescent reporters in whole-animal settings like in the nematode