Produção Nacional
Revisado por pares
IgY antibody and human neurocysticercosis: a novel approach on immunodiagnosis using Taenia crassiceps hydrophobic antigens
2019; Cambridge University Press; Volume: 147; Issue: 2 Linguagem: Inglês
10.1017/s0031182019001446
ISSN1469-8161
AutoresGuilherme Carrara Moreira Paiva, Gabriela B. Silva, Lucas Silva de Faria, Daniela Nunes, Vanessa da Silva Ribeiro, Camila de Almeida Lopes, Maria do Rosário F. Gonçalves-Pires, Malú Mateus Santos Obata, Isabela Pacheco Borges, Álvaro Ferreira Júnior, Veridiana de Melo Rodrigues, Julia Maria Costa‐Cruz,
Tópico(s)Parasites and Host Interactions
ResumoAbstract Human neurocysticercosis (NCC) is a worldwide neglected disease caused by Taenia solium metacestode and responsible for various complications and neurological disorders. This study aimed to evaluate the use of specific immunoglobulin Y (IgY) produced by laying hens immunized with a hydrophobic fraction of Taenia crassiceps metacestodes (hF Tc ) in NCC diagnosis. Egg yolk IgY antibodies were fractionated, purified and characterized. Enzyme-linked immunosorbent assay (ELISA) was carried out to evaluate the production kinetics and avidity maturation of anti-hF Tc IgY antibodies throughout the IgY obtention process. Antigen recognition tests were carried out by Western blotting and immunofluorescence antibody test using purified and specific anti-hF Tc IgY antibodies for detection of parasitic antigens of T. crassiceps and T. solium metacestodes. Sandwich ELISA was performed to detect circulating immune complexes formed by IgG and parasitic antigens in human sera. The results showed high diagnostic values (93.2% sensitivity and 94.3% specificity) for immune complexes detection in human sera with confirmed NCC. In conclusion, specific IgY antibodies produced from immunized hens with hF Tc antigens were efficient to detect T. solium immune complexes in human sera, being an innovative and potential tool for NCC immunodiagnosis.
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