Portal de Periódicos da CAPES

Artigo Acesso aberto Produção Nacional Revisado por pares

BIBTEX RIS

Oral gallic acid improves metabolic profile by modulating SIRT1 expression in obese mice brown adipose tissue: A molecular and bioinformatic approach

2019; Elsevier BV; Volume: 237; Linguagem: Inglês

10.1016/j.lfs.2019.116914

1879-0631

Alanna Fernandes Paraíso, Jaciara Neves Sousa, João Marcus Oliveira Andrade, Eloá Santos Mangabeira, Deborah de Farias Lelis, Alfredo Maurício Batista de Paula, Andréia Maria Eleutério Barros-Lima Martins, William James Nogueira Lima, André Luiz Sena Guimarães, Geraldo Aclécio Melo, Michaela Schwarz, Sérgio Henrique Sousa Santos,

Biochemical effects in animals

The aim of the presente study was to examine the effects of oral gallic acid (GA) administration on the brown adipose tissue of obese mice fed with high-fat diet. New mechanisms and interactions pathways in thermogenesis were accessed through bioinformatics analyses.Swiss male mice were divided into four groups and fed during 60 days with: standard diet, standard diet combined with gallic acid, high-fat diet and high-fat diet combined with gallic acid. Body weight, food intake, and blood parameters (glucose tolerance test, total-cholesterol, high-density low-c, triglyceride and glucose levels) were evaluated. Brown and subcutaneous white adipose tissue histological analysis were performed. SIRT1 and PGC1-α mRNA expression in the brown adipose tissue were assessed.Our main findings showed that the gallic acid improved glucose tolerance and metabolic parameters. These results were accompanied by bioinformatics analyses that evidenced SIRT1 as main target in the thermogenesis process, confirmed as increased SIRT1 mRNA expression was evidenced in the brown adipose tissue.Together, the data suggest that the gallic acid effect in brown adipose tissue may improve body metabolism, glucose homeostasis and increase thermogenesis.