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Blue laser light inhibits biofilm formation in vitro and in vivo by inducing oxidative stress

2019; Nature Portfolio; Volume: 5; Issue: 1 Linguagem: Inglês

10.1038/s41522-019-0102-9

2055-5008

Katia Rupel, Luisa Zupin, Giulia Ottaviani, Iris Bertani, Valentina Martinelli, Davide Porrelli, Simone Vodret, Roman Vuerich, Daniel Passos da Silva, Rossana Bussani, Sérgio Crovella, Matthew R. Parsek, Vittorio Venturi, Roberto Di Lenarda, Matteo Biasotto, Serena Zacchigna,

Connexins and lens biology

Resolution of bacterial infections is often hampered by both resistance to conventional antibiotic therapy and hiding of bacterial cells inside biofilms, warranting the development of innovative therapeutic strategies. Here, we report the efficacy of blue laser light in eradicating Pseudomonas aeruginosa cells, grown in planktonic state, agar plates and mature biofilms, both in vitro and in vivo, with minimal toxicity to mammalian cells and tissues. Results obtained using knock-out mutants point to oxidative stress as a relevant mechanism by which blue laser light exerts its anti-microbial effect. Finally, the therapeutic potential is confirmed in a mouse model of skin wound infection. Collectively, these data set blue laser phototherapy as an innovative approach to inhibit bacterial growth and biofilm formation, and thus as a realistic treatment option for superinfected wounds.