Oyster mortality
2015; Wiley; Volume: 13; Issue: 6 Linguagem: Inglês
10.2903/j.efsa.2015.4122
ISSN1831-4732
Tópico(s)Child Nutrition and Water Access
ResumoEFSA JournalVolume 13, Issue 6 4122 OpinionOpen Access Oyster mortality EFSA Panel on Animal Health and Welfare (AHAW), EFSA Panel on Animal Health and Welfare (AHAW)Search for more papers by this author EFSA Panel on Animal Health and Welfare (AHAW), EFSA Panel on Animal Health and Welfare (AHAW)Search for more papers by this author First published: 04 June 2015 https://doi.org/10.2903/j.efsa.2015.4122Citations: 26 Panel members: Charlotte Berg, Anette Bøtner, Howard Browman, Aline De Koeijer, Klaus Depner, Mariano Domingo, Christian Ducrot, Sandra Edwards, Christine Fourichon, Frank Koenen, Simon More, Mohan Raj, Liisa Sihvonen, Hans Spoolder, Jan Arend Stegeman, Hans-Hermann Thulke, Ivar Vågsholm, Antonio Velarde and Preben Willeberg Correspondence: [email protected] Acknowledgement: The Panel wishes to thank the members of the Working Group on oyster mortality: Howard Browman, Celine Garcia, Ron Lee, Stein Mortensen, Fabrice Pernet, Ana Roque, Anne Thebault and EFSA staff members: Per Have and Frank Verdonck for the support provided to this scientific output. Adoption date: 5 May 2015 Published date: 4 June 2015 Question number: EFSA-Q-2014-00188 On request from: European Commission AboutPDF ToolsExport citationAdd to favoritesTrack citation ShareShare Give accessShare full text accessShare full-text accessPlease review our Terms and Conditions of Use and check box below to share full-text version of article.I have read and accept the Wiley Online Library Terms and Conditions of UseShareable LinkUse the link below to share a full-text version of this article with your friends and colleagues. Learn more.Copy URL Abstract This updated scientific opinion on oyster mortality addresses 1) the role of ostreid herpesvirus (OsHV-1) in mortality, 2) evidence for a role of Vibrio aestuarianus in mortality, 3) effectiveness of water treatment in inactivating OsHV-1 and V. aestuarianus and 4) feasibility, availability and effectiveness of the disease prevention and control measures. A new pattern of mass mortality of Pacific oysters (C. gigas) was observed in France and other European countries from 2008 onwards. Similar outbreaks were reported in 2010-11 from Australia and New Zealand. Studies performed since 2010 has provided strong evidence that OsHV-1 µVar is causally linked to increased oyster spat mortality at sea water temperatures above 16 °C. V. aestuarianus subsp. francensis was detected in France in 2001 in moribund oysters; since then this bacterium has been regularly detected during oyster mortality events. Owing to a lack of information, the causal relationship between V. aestuarianus and oyster mortality has not been established. Discharge of untreated seawater from depuration plants remains a potential mode of transmission of diseases affecting bivalves and other marine life. Effective disinfection of seawater effluent from depuration and holding facilities will minimize the risk of transmission of infectious agents. Unrestricted movement of oysters is associated with a high risk of spread of OsHV-1. Wild populations of C. gigas also contribute to spread of OsHV-1. Only a few areas in Europe continue to remain free from OsHV-1. Once infected, an area is not likely to regain freedom from OsHV-1 if a wild population of C. gigas is present. Almost all OsHV-1 strains isolated after 2008 conform to the definition of microvariants. Therefore, it appears unnecessary to maintain a separate definition of microvariants for disease control purposes. The criteria in Directive 2006/88/EC for listing of non-exotic diseases are currently not fulfilled for mortality caused by OsHV-1 microvariants. References Aboubaker MH, Sabrie J, Huet M and Koken M, 2013. Establishment of stable GFP-tagged Vibrio aestuarianus strains for the analysis of bacterial infection-dynamics in the Pacific oyster, Crassostrea gigas. Veterinary Microbiology, 164, 392– 398. Allen Jr SK and Downing SL, 1986. Performance of triploid Pacific oysters, Crassostrea gigas (Thunberg). I. Survival, growth, glycogen content, and sexual maturation in yearlings. Journal of Experimental Marine Biology and Ecology, 102, 197– 208. Andrews LS, Park DL and Chen YP, 2000. Low temperature pasteurization to reduce the risk of Vibrio infections from raw shell-stock oysters. Food Additives & Contaminants, 17, 787– 791. Aranguren R, Costa MM, Novoa B and Figueras A, 2012. Detection of herpesvirus variant (OsHV-1 mu var) in Pacific oysters (Crassostrea gigas) in Spain and development of a rapid method for its differential diagnosis. Bulletin of the European Association of Fish Pathologists, 32, 24– 29. Arzul I, Renault T and Lipart C, 2001a. Experimental herpes-like viral infections in marine bivalves: demonstration of interspecies transmission. Diseases of Aquatic Organisms, 46, 1– 6. Arzul I, Renault T, Lipart C and Davison AJ, 2001b. Evidence for interspecies transmission of oyster herpesvirus in marine bivalves. Journal of General Virology, 82, 865– 870. Azandegbe A, Garnier M, Andrieux-Loyer F, Kerouel R, Philippon X and Nicolas JL, 2010. Occurrence and seasonality of Vibrio aestuarianus in sediment and Crassostrea gigas haemolymph at two oyster farms in France. Diseases of Aquatic Organisms, 91, 213– 221. Bai C, Wang C, Xia J, Sun H, Zhang S and Huang J, 2015. Emerging and endemic types of Ostreid herpesvirus 1 were detected in bivalves in China. Journal of Invertebrate Pathology, 124, 98– 106. Balbi T, Fabbri R, Cortese K, Smerilli A, Ciacci C, Grande C, Vezzulli L, Pruzzo C and Canesi L, 2013. Interactions between Mytilus galloprovincialis hemocytes and the bivalve pathogens Vibrio aestuarianus 01/032 and Vibrio splendidus LGP32. Fish & Shellfish Immunology, 35, 1906– 1915. Batista FM, López-Sanmartín M, Boudry P, Navas JI, Ruano F, Renault T, Fonseca VG and Leitão A, 2014. Insights on the association between somatic aneuploidy and ostreid herpesvirus 1 detection in the oysters Crassostrea gigas, C. angulata and their F1 hybrids. Aquaculture Research. Available online: http://onlinelibrary.wiley.com/doi/10.1111/are.12613/full. Batista FM, López-Sanmartín M, Grade A, Morgado I, Valente M, Navas JI, Power DM and Ruano F, 2015. Sequence variation in ostreid herpesvirus 1 microvar isolates detected in dying and asymptomatic Crassostrea angulata adults in the Iberian Peninsula: Insights into viral origin and spread. Aquaculture, 435, 43– 51. Baulch T, Ellard K and Bradshaw M, 2013. Tasmanian Abalone Biosecurity Project: Implementation Phase 1: Biosecurity Strategies for Abalone Processors. Journal of Shellfish Research, 32, 33– 35. Beukema JJ and Dekker R, 2010. Extraordinarily low mortality rates reported in juvenile Pacific oysters in the German Wadden Sea. Helgoland Marine Research, 64, 69– 70. Bingham P, Brangenberg N, Williams R and Andel Mv, 2013. Marine and freshwater investigation into the first diagnosis of ostreid herpesvirus type 1 in Pacific oysters. Surveillance (Wellington), 40, 20– 24. BIVALIFE, 2014. Controlling infectious diseases in oysters and mussels in Europe. Final dissemination report. Available online: http://www.bivalife.eu/content/download/79799/1014051/file/BIVALIFE%20-%20Deliverable%20D6%207%20-%20Final%20dissemination%20report.pdf. Burge CA and Friedman CS, 2012. Quantifying ostreid herpesvirus (OsHV-1) genome copies and expression during transmission. Microbial Ecology, 63, 596– 604. Burge CA, Griffin FJ and Friedman CS, 2006. Mortality and herpesvirus infections of the Pacific oyster Crassostrea gigas in Tomales Bay, California, USA. Diseases of Aquatic Organisms, 72, 31– 43. Chen M, Tan Z and Zeng G, 2011. Microsatellite is an important component of complete Hepatitis C virus genomes. Infection, Genetics and Evolution, 11, 1646– 1654. Cheney DP, Elston RA, MacDonald BF, Cherr GN, Hamdoun AM and Jacobsen JL, 2000. An update on the ongoing oyster summer mortality study: Mortality of the pacific oyster, Crassostrea gigas: Health screening, environmental links and management options. Journal of Shellfish Research, 19, 353– 359. Clegg TA, Morrissey T, Geoghegan F, Martin SW, Lyons K, Ashe S and More SJ, 2014. Risk factors associated with increased mortality of farmed Pacific oysters in Ireland during 2011. Preventive Veterinary Medicine, 113, 257– 267. Corbeil S, Williams LM, Bergfeld J and Crane MSJ, 2012. Abalone herpes virus stability in sea water and susceptibility to chemical disinfectants. Aquaculture, 326, 20– 26. Corporeau C, Tamayo D, Pernet F, Quéré C and Madec S, 2014. Proteomic signatures of the oyster metabolic response to herpesvirus OsHV-1 $Var infection. Journal of Proteomics, 109, 176– 187. Davis CL, Field D, Metzgar D, Saiz R, Morin PA, Smith IL, Spector SA and Wills C, 1999. Numerous length polymorphisms at short tandem repeats in human cytomegalovirus. Journal of Virology, 73, 6265– 6270. Davison A, Trus B, Cheng N, Steven A, Watson M, Cunningham C, Le Deuff R and Renault T, 2005. A novel class of herpesvirus with bivalve hosts. Journal of General Virology, 86, 41– 53. Davison AJ, Eberle R, Ehlers B, Hayward GS, McGeoch DJ, Minson AC, Pellett PE, Roizman B, Studdert MJ and Thiry E, 2009. The order Herpesvirales. Archives of Virology, 154, 171– 177. De Decker S, Normand J, Saulnier D, Pernet F, Castagnet S and Boudry P, 2011. Responses of diploid and triploid Pacific oysters Crassostrea gigas to Vibrio infection in relation to their reproductive status. Journal of Invertebrate Pathology, 106, 179– 191. De Decker S and Saulnier D, 2011. Vibriosis induced by experimental cohabitation in Crassostrea gigas: Evidence of early infection and down-expression of immune-related genes. Fish & Shellfish Immunology, 30, 691– 699. Deback C, Boutolleau D, Depienne C, Luyt CE, Bonnafous P, Gautheret-Dejean A, Garrigue I and Agut H, 2009. Utilization of microsatellite polymorphism for differentiating herpes simplex virus type 1 strains. Journal of Clinical Microbiology, 47, 533– 540. Dégremont L, 2011. Evidence of herpesvirus (OsHV-1) resistance in juvenile Crassostrea gigas selected for high resistance to the summer mortality phenomenon. Aquaculture, 317, 94– 98. Dügremont L, 2013. Size and genotype affect resistance to mortality caused by OsHV-1 in Crassostrea gigas. Aquaculture, 416–417, 129– 134. Dügremont L, Azéma P and Travers MA, 2014a. Spat and adult mortality related to Vibrio aestuarianus in Crassostrea gigas in France. Proceedings of the National Shellfish Association Meeting, Jacksonville, Florida, USA, March 29–April 2, 2014. Dügremont L, Bédier E and Boudry P, 2010. Summer mortality of hatchery-produced Pacific oyster spat (Crassostrea gigas). II. Response to selection for survival and its influence on growth and yield. Aquaculture, 299, 21– 29. Dügremont L and Benabdelmouna A, 2014. Mortality associated with OsHV-1 in spat Crassostrea gigas: role of wild-caught spat in the horizontal transmission of the disease. Aquaculture International, 1– 15. Dügremont L, Ernande B, Bedier E and Boudry P, 2007. Summer mortality of hatchery-produced Pacific oyster spat (Crassostrea gigas). I. Estimation of genetic parameters for survival and growth. Aquaculture, 262, 41– 53. Dégremont L, Ledu C, Maurouard E, Nourry M and Benabdelmouna A, 2014b. Effect of ploidy on the mortality of Crassostrea gigas spat caused by OsHV-1 in France using unselected and selected OsHV-1 resistant oysters. Aquaculture Research, n/a-n/a. Diederich S, 2006. High survival and growth rates of introduced Pacific oysters may cause restrictions on habitat use by native mussels in the Wadden Sea. Journal of Experimental Marine Biology and Ecology, 328, 211– 227. Dieu BTM, Marks H, Zwart MP and Vlak JM, 2010. Evaluation of white spot syndrome virus variable DNA loci as molecular markers of virus spread at intermediate spatiotemporal scales. Journal of General Virology, 91, 1164– 1172. Dolmer P, Holm MW, Strand Å, Lindegarth S, Bodvin T, Norling P and Mortensen S, 2014. The invasive Pacific oyster, Crassostrea gigas, in Scandinavian coastal waters: A risk assessment on the impact in different habitats and climate conditions. Fisken og Havet, 2. Domeneghetti S, Varotto L, Civettini M, Rosani U, Stauder M, Pretto T, Pezzati E, Arcangeli G, Turolla E, Pallavicini A and Venier P, 2014. Mortality occurrence and pathogen detection in Crassostrea gigas and Mytilus galloprovincialis close-growing in shallow waters (Goro lagoon, Italy). Fish & Shellfish Immunology. Dominguez M, François C, Lupo C, Rautureau S and Calavas D, 2013. Mortalité d'huîtres creuses adultes en 2012 : Bilan des déclarations des professionnels auprès d'un échantillon de DDTM et données du Repamo, RESCO et SUMO. Plateforme nationale de surveillance épidémiologique en santé animale. Available online: http://plateforme-esa.fr/index.php?option=com_content&view=category&layout=blog&id=89&Itemid=232. Du Y, Zhang L, Xu F, Huang B, Zhang G and Li L, 2013. Validation of housekeeping genes as internal controls for studying gene expression during Pacific oyster (Crassostrea gigas) development by quantitative real-time PCR. Fish & Shellfish Immunology, 34, 939– 945. EFSA Panel on Animal Health and Welfare (AHAW)., 2010. Scientific Opinion on a request from the European Commission on the increased mortality events in Pacific oysters Crassostrea gigas. EFSA Journal, 8(11): 1894. 60 pp. doi:10.2903/j.efsa.2010.1894. EFSA Panel on Biological Hazards (BIOHAZ) and EFSA Panel on Contaminants in the Food Chain (CONTAM), 2012. Scientific Opinion on the minimum hygiene criteria to be applied to clean seawater and on the public health risks and hygiene criteria for bottled seawater intended for domestic use. EFSA Journal, 10(3): 2613. [ 85 pp.] doi:10.2903/j.efsa.2012.2613. Eiler A, Johansson M and Bertilsson S, 2006. Environmental influences on Vibrio populations in northern temperate and boreal coastal waters (Baltic and Skagerrak Seas). Applied and Environmental Microbiology, 72, 6004– 6011. Engelsma MY, 2012. Verslag monitoring oester herpesvirus 2011; Resultaten van onderzoek naar de aanwezigheid van oester herpesvirus in Japanse oesters van het Grevelingenmeer en de Oosterschelde. CVI-rapport 12/CVI0098. Centraal Veterinair Instituut, Lelystad [In Dutch]. FAO and WHO, 2012. Code of practice for fish and fishery products. 2. ed., Codex Alimentarius Commission, 243 pp. Farley C, Banfield W, Kasnic G and Foster W, 1972. Oyster herpes-type virus. Science, 178, 759– 760. Fleury E, Jolivet A, Mazurié J, Cochennec Laureau N and Bédier E, 2014. Spatio-temporal evolution of summer mortalities affecting spat of Pacific oysters Crassostrea gigas in French farming areas. Proceedings of the Proceedings of the 106th Annual Meeting of National Shellfisheries Association, Jacksonville, Florida, USA. François C, Joly JP, Garcia C, Lupo C, Travers MA, Pépin J-F, Hatt PJ, Arzul I, Omnes E, Tourbiez D, Faury N, Haffner P, Huchet E, Dubreuil C, Chollet B, Renault T, Cordier R, Hebert P, Le Gagneur E, Parrad S, Gerla D, Annezo J-P, Terre-Terrillon A, Le Gal D, Langlade A, Bédier E, Hittier B, Grizon J, Chabirand J-M, Robert S, Seugnet J-L, Rumebe M, Le Gall P, Bouchoucha M, Baldi Y and Masson J-C 2013. Bilan 2012 du réseau Repamo. Available from: http://wwz.ifremer.fr/repamo/Documentation/Rapports-d-activites. Friedman CS, Estes RM, Stokes NA, Burge CA, Hargove JS, Barber BJ, Elston RA, Burreson EM and Reece KS, 2005. Herpes virus in juvenile Pacific oysters Crassostrea gigas from Tomales Bay, California, coincides with summer mortality episodes. Diseases of Aquatic Organisms, 63, 33– 41. García-Aljaro C, Vargas-Cespedes GJ and Blanch AR, 2012. Detection of acylated homoserine lactones produced by Vibrio spp. and related species isolated from water and aquatic organisms. Journal of Applied Microbiology, 112, 383– 389. Garcia C, Arzul I, Chollet B, Robert M, Omnes E, Ferrand S, Faury N, Tourbiez D, Haffner P, Miossec L, Joly JP and François C, 2014. Vibrio aestuarianus and Pacific oysters, Crassostrea gigas mortality in France: a new chapter in their relation. Proceedings of the National Shellfish Association, Jacksonville, Florida, USA. Garcia C, Thebault A, Degremont L, Arzul I, Miossec L, Robert M, Chollet B, Francois C, Joly JP, Ferrand S, Kerdudou N and Renault T, 2011. Ostreid herpesvirus 1 detection and relationship with Crassostrea gigas spat mortality in France between 1998 and 2006. Veterinary Research, 42, 73. Garnier M, Labreuche Y, Garcia C, Robert A and Nicolas JL, 2007. Evidence for the involvement of pathogenic bacteria in summer mortalities of the Pacific oyster Crassostrea gigas. Microbial Ecology, 53, 187– 196. Garnier M, Labreuche Y and Nicolas J-L, 2008. Molecular and phenotypic characterization of Vibrio aestuarianus subsp francensis subsp nov., a pathogen of the oyster Crassostrea gigas. Systematic and Applied Microbiology, 31, 358– 365. Gittenberger A, Voorbergen-Laarman MA and Engelsma MY, 2015. Ostreid herpesvirus OsHV-1 μVar in Pacific oysters Crassostrea gigas (Thunberg 1793) of the Wadden Sea, a UNESCO world heritage site. Journal of Fish Diseases. doi: 10.1111/jfd.12332. Goudenège D, Travers MA, Lemire A, Haffner P, Labreuche Y, Petton B, Mangenot S, Calteau A, Mazel D, Nicolas JL, Jacq A and Le Roux F, 2015. A single regulatory gene is sufficient to alter Vibrio aestuarianus pathogenicity in oysters. Environmental Microbiology, in press. doi: 10.1111/1462–2920.12699. Gray RJH and Hsu DH-L, 1979. Effectiveness of iodophor in the destruction of Vibrio Parahaemolyticus. Journal of Food Science, 44, 1097– 1100. Green TJ, Robinson N, Chataway T, Benkendorff K, O'Connor W and Speck P, 2014. Evidence that the major hemolymph protein of the Pacific oyster, Crassostrea gigas, has antiviral activity against herpesviruses. Antiviral Research, 110, 168– 174. Grijalva-Chon JM, Castro-Longoria R, Ramos-Paredes J, Enríquez-Espinoza TL and Mendoza-Cano F, 2013. Detection of a new OsHV-1 DNA strain in the healthy Pacific oyster, Crassostrea gigas Thunberg, from the Gulf of California. Journal of Fish Diseases, 36, 965– 968. Guisande JA, Montes M, Farto R, Armada SP, Perez MJ and Nieto TP, 2004. A set of tests for the phenotypic identification of culturable bacteria associated with Galician bivalve mollusc production. Journal of Shellfish Research, 23, 599– 609. Hijnen WAM, Brouwer-Hanzens AJ, Charles KJ and Medema GJ, 2005. Transport of MS2 phage, Escherichia coli, Clostridium perfringens, Cryptosporidium parvum, and Giardia intestinalis in a gravel and a sandy soil. Environmental Science & Technology, 39, 7860– 7868. Hill AB, 1965. The Environment and Disease: Association or Causation? Proceedings of the Royal Society of Medicine, 58, 295– 300. Hooper C, Hardy-Smith P and Handlinger J, 2007. Ganglioneuritis causing high mortalities in farmed Australian abalone (Haliotis laevigata and Haliotis rubra). Australian Veterinary Journal, 85, 188– 193. Houng HS, Lott L, Gong H, Kuschner RA, Lynch JA and Metzgar D, 2009. Adenovirus microsatellite reveals dynamics of transmission during a recent epidemic of human adenovirus serotype 14 infection. Journal of Clinical Microbiology, 47, 2243– 2248. Hoyer O, 1998. Testing performance and monitoring of UV systems for drinking water disinfection. Water Supply, 16, 424– 429. Hsieh JL, Fries JS and Noble RT, 2008. Dynamics and predictive modelling of Vibrio spp. in the Neuse River Estuary, North Carolina, USA. Environmental Microbiology, 10, 57– 64. Hwang JY, Park JJ, Yu HJ, Hur YB, Arzul I, Couraleau Y and Park MA, 2013. Ostreid herpesvirus 1 infection in farmed Pacific oyster larvae Crassostrea gigas (Thunberg) in Korea. Journal of Fish Diseases, 36, 969– 972. Jee BY, Lee SJ, Cho MY, Lee SJ, Kim JW, Choi SH, Jeong HD and Kim KH, 2013. Detection of Ostreid Herpesvirus 1 from adult Pacific Oysters Crassostrea gigas Cultured in Korea. Fisheries and Aquatic Sciences, 16, 131– 135. Jenkins C, Hick P, Gabor M, Spiers Z, Fell SA, Gu X, Read A, Go J, Dove M, O'Connor W, Kirkland PD and Frances J, 2013. Identification and characterisation of an ostreid herpesvirus-1 microvariant (OsHV-1 mu-var) in Crassostrea gigas (Pacific oysters) in Australia. Diseases of Aquatic Organisms, 105, 109– 126. Junli H, Li W, Nenqi R, Li LX, Fun SR and Guanle Y, 1997. Disinfection effect of chlorine dioxide on viruses, algae and animal planktons in water. Water Research, 31, 455– 460. Kasai H, Muto Y and Yoshimizu M, 2005. Virucidal effects of ultraviolet, heat treatment and disinfectants against koi herpesvirus (KHV). Fish Pathology, 40, 137– 138. Keeling SE, Brosnahan CL, Williams R, Gias E, Hannah M, Bueno R, McDonald WL and Johnston C, 2014. New Zealand juvenile oyster mortality associated with ostreid herpesvirus 1-an opportunistic longitudinal study. Diseases of Aquatic Organisms, 109, 231– 239. Krkošek M, 2010. Review: Host density thresholds and disease control for fisheries and aquaculture. Aquacult Environ Interact, 1, 21– 32. Labreuche Y, Lambert C, Soudant P, Boulo V, Huvet A and Nicolas J-L, 2006a. Cellular and molecular hemocyte responses of the Pacific oyster, Crassostrea gigas, following bacterial infection with Vibrio aestuarianus strain 01/32. Microbes and Infection, 8, 2715– 2724. Labreuche Y, Le Roux F, Henry J, Zatylny C, Huvet A, Lambert C, Soudant P, Mazel D and Nicolas J-L, 2010. Vibrio aestuarianus zinc metalloprotease causes lethality in the Pacific oyster Crassostrea gigas and impairs the host cellular immune defenses. Fish & Shellfish Immunology, 29, 753– 758. Labreuche Y, Soudant P, Goncalves M, Lambert C and Nicolas JL, 2006b. Effects of extracellular products from the pathogenic Vibrio aestuarianus strain 01/32 on lethality and cellular immune responses of the oyster Crassostrea gigas. Developmental and Comparative Immunology, 30, 367– 379. Le Deuff R-M, Nicolas J-L, Renault T and Cochennec N, 1994. Experimental transmission of a Herpes-like virus to axenic larvae of Pacific oyster, Crassostrea gigas. Bulletin of the European Association of Fish Pathologists, 14, 69– 72. Le Deuff R and Renault T, 1999. Purification and partial genome characterization of a herpes-like virus infecting the Japanese oyster, Crassostrea gigas. Journal of General Virology, 80, 1317– 1322. Le Deuff RM, Renault T and Gerard A, 1996. Effects of temperature on herpes-like virus detection among hatchery-reared larval Pacific oyster Crassostrea gigas. Diseases of Aquatic Organisms, 24, 149– 157. Le Roux F, Labreuche Y, Davis BM, Iqbal N, Mangenot S, Goarant C, Mazel D and Waldor MK, 2011. Virulence of an emerging pathogenic lineage of Vibrio nigripulchritudo is dependent on two plasmids. Environmental Microbiology, 13, 296– 306. Lee R, Lovatelli A and Ababouch L, 2008. Bivalve depuration: fundamental and practical aspects. FAO Fisheries Technical Paper. No. 511. Rome, FAO. 2008. 139pp. Lelie PN, Reesink HW and Lucas CJ, 1987. Inactivation of 12 viruses by heating steps applied during manufacture of a hepatitis B vaccine. Journal of Medical Virology, 23, 297– 301. Lewis T, Defenderfer D and Zippel B 2012. Understanding and planning for the potential impacts of OsHV-1 μVar on the Australian Pacific oyster industry. Project No: FRDC 2011/043. Available online: http://oysterstasmania.org/wp-content/uploads/2013/09/potentional-impact-oshv-1–2011–043-DLD.pdf. Lin M-H, Tsai T-Y, Hsieh S-C, Yu R-C and Chou C-C, 2013. Susceptibility of Vibrio parahemolyticus to disinfectants after prior exposure to sublethal stress. Food Microbiology, 34, 202– 206. Lynch S, Carlsson J, Reilly A, Cotter E and Culloty S, 2012. A previously undescribed ostreid herpes virus 1 (OsHV-1) genotype detected in the pacific oyster, Crassostrea gigas, in Ireland. Parasitology, 139, 1526– 1532. Lynch SA, Dillane E, Carlsson J and Culloty SC, 2013. Development and assessment of a sensitive and cost-effective polymerase chain reaction to detect ostreid herpesvirus 1 and variants. Journal of Shellfish Research, 32, 657– 664. Martenot C, Denechère L, Hubert P, Metayer L, Oden E, Trancart S, Travaillé E and Houssin M, 2015. Virulence of Ostreid herpesvirus 1 μVar in sea water at 16 °C and 25 °C. Aquaculture, 439, 1– 6. Martenot C, Fourour S, Oden E, Jouaux A, Travaille E, Malas JP and Houssin M, 2012. Detection of the OsHV-1μVar in the Pacific oyster Crassostrea gigas before 2008 in France and description of two new microvariants of the Ostreid Herpesvirus 1 (OsHV-1). Aquaculture, 338, 293– 296. Martenot C, Oden E, Travaillé E, Malas J-P and Houssin M, 2011. Detection of different variants of Ostreid Herpesvirus 1 in the Pacific oyster, Crassostrea gigas between 2008 and 2010. Virus Research, 160, 25– 31. Martenot C, Travaillé E, Lethuillier O, Lelong C and Houssin M, 2013. Genome exploration of six variants of the Ostreid Herpesvirus 1 and characterization of large deletion in OsHV-1$Var specimens. Virus Research, 178, 462– 470. McCallum HI, Kuris A, Harvell CD, Lafferty KD, Smith GW and Porter J, 2004. Does terrestrial epidemiology apply to marine systems? Trends in Ecology & Evolution, 19, 585– 591. McCleary S and Henshilwood K, 2015. Novel quantitative TaqMan® MGB real time PCR for sensitive detection of Vibrio aestuarianus in Crassostrea gigas. Diseases of Aquatic Organisms. Mineur F, Provan J and Arnott G, 2015. Phylogeographical analyses of shellfish viruses: inferring a geographical origin for ostreid herpesviruses OsHV-1 (Malacoherpesviridae). Marine Biology, 162, 181– 192. Montes M, Farto R, Perez MJ, Nieto TP, Larsen JL and Christensen H, 2003. Characterization of Vibrio strains isolated from turbot (Scophthalmus maximus) culture by phenotypic analysis, ribotyping and 16S rRNA gene sequence comparison. J Appl Microbiol, 95, 693– 703. Morrissey T, McCleary S, Collins E, Henshilwood K and Cheslett D, 2015. An investigation of Ostried Herpes Virus microvariants found in Crassostrea gigas oyster producing bays in Ireland. Aquaculture 442, 86– 92. Mortensen S, Bodvin T, Skår CK, Sælemyr L, Jelmert A, Albretsen J and Naustvoll LJ (Institute of Marine Research), 2014. Massedød av stillehavsøsters, Crassostrea gigas, i Sverige og Norge, september 2014. Undersøkelser av østers fra Vestfold og funn av østers herpesvirus (OsHV-1 μvar). Rapport Fra Havforskningen, 28– 2014. Murray AG, Marcos-Lopez M, Collet B and Munro LA, 2012. A review of the risk posed to Scottish mollusc aquaculture from Bonamia, Marteilia and oyster herpesvirus. Aquaculture, 370–371, 7– 13. Nell JA, 2002. Farming triploid oysters. Aquaculture, 210, 69– 88. Nhung PH, Shah MM, Ohkusu K, Noda M, Hata H, Sun XS, Iihara H, Goto K, Masaki T, Miyasaka J and Ezaki T, 2007. The dnaJ gene as a novel phylogenetic marker for identification of Vibrio species. Systematic and Applied Microbiology, 30, 309– 315. Normand J, Li R, Quillien V, Nicolas J-L, Boudry P, Pernet F and Huvet A, 2014. Contrasted survival under field or controlled conditions displays associations between mRNA levels of candidate genes and response to OsHV-1 infection in the Pacific oyster Crassostrea gigas. Marine Genomics, 15, 95– 102. Oden E, Martenot C, Berthaux M, Travaillé E, Malas JP and Houssin M, 2011. Quantification of ostreid herpesvirus 1 (OsHV-1) in Crassostrea gigas by real-time PCR: Determination of a viral load threshold to prevent summer mortalities. Aquaculture, 317, 27– 31. Paul-Pont I, Dhand NK and Whittington RJ, 2013a. Influence of husbandry practices on OsHV-1 associated mortality of Pacific oysters Crassostrea gigas. Aquaculture, 412–413, 202– 214. Paul-Pont I, Dhand NK and Whittington RJ, 2013b. Spatial distribution of mortality in Pacific oysters Crassostrea gigas: reflection on mechanisms of OsHV-1 transmission. Diseases of Aquatic Organisms, 105, 127– 138. Paul-Pont I, Evans O, Dhand NK, Rubio A, Coad P and Whittington RJ, 2014. Descriptive epidemiology of mass mortality due to Ostreid herpesvirus-1 (OsHV-1) in commercially farmed Pacific oysters (Crassostrea gigas) in the Hawkesbury River estuary, Australia. Aquaculture, 422–423, 146– 159. Paul-Pont I, Evans O, Dhand NK and Whittington RJ, 2015. Experimental infections of Pacific oyster
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