Produção Nacional
Revisado por pares
Identification of Physalis angulata (Solanaceae) as a Natural Alternative Weed Host of Tomato Severe Rugose Virus in Brazil
2019; American Phytopathological Society; Volume: 104; Issue: 2 Linguagem: Inglês
10.1094/pdis-07-19-1389-pdn
ISSN1943-7692
AutoresMacária Ferreira Duarte, M. E. N. Fonseca, L. S. Boiteux, Hélcio Costa, Bergmann Morais Ribeiro, Fernando L. Melo, R. C. Pereira–Carvalho,
Tópico(s)Plant Disease Management Techniques
ResumoHomePlant DiseaseVol. 104, No. 2Identification of Physalis angulata (Solanaceae) as a Natural Alternative Weed Host of Tomato Severe Rugose Virus in Brazil PreviousNext DISEASE NOTES OPENOpen Access licenseIdentification of Physalis angulata (Solanaceae) as a Natural Alternative Weed Host of Tomato Severe Rugose Virus in BrazilM. F. Duarte, M. E. N. Fonseca, L. S. Boiteux, H. Costa, B. M. Ribeiro, F. L. Melo, and R. C. Pereira-CarvalhoM. F. Duartehttp://orcid.org/0000-0002-1931-9594Plant Pathology Department, University of Brasilia, Brasilia, DF, Brazil, M. E. N. FonsecaEmbrapa Vegetable Crops (CNPH), Brasilia, DF, Brazil, L. S. Boiteuxhttp://orcid.org/0000-0002-5791-8002Embrapa Vegetable Crops (CNPH), Brasilia, DF, Brazil, H. CostaINCAPER, Venda Nova do Imigrante, ES, Brazil, B. M. RibeiroCell Biology Department, University of Brasilia, Brasilia, DF, Brazil, F. L. MeloPlant Pathology Department, University of Brasilia, Brasilia, DF, Brazil, and R. C. Pereira-Carvalho†Corresponding author: R. C. Pereira-Carvalho; E-mail Address: carvalhorcp@gmail.comhttp://orcid.org/0000-0002-9905-189XPlant Pathology Department, University of Brasilia, Brasilia, DF, Brazil AffiliationsAuthors and Affiliations M. F. Duarte1 M. E. N. Fonseca2 L. S. Boiteux2 H. Costa3 B. M. Ribeiro4 F. L. Melo1 R. C. Pereira-Carvalho1 † 1Plant Pathology Department, University of Brasilia, Brasilia, DF, Brazil 2Embrapa Vegetable Crops (CNPH), Brasilia, DF, Brazil 3INCAPER, Venda Nova do Imigrante, ES, Brazil 4Cell Biology Department, University of Brasilia, Brasilia, DF, Brazil Published Online:4 Dec 2019https://doi.org/10.1094/PDIS-07-19-1389-PDNAboutSectionsSupplemental ToolsAdd to favoritesDownload CitationsTrack Citations ShareShare onFacebookTwitterLinked InRedditEmailWechat A diverse array of Begomovirus species (family Geminiviridae) can induce economically important diseases in all major tomato-producing areas in Brazil (Fernandes et al. 2008). Weeds of the Solanaceae family have been identified as important alternative hosts of the viral species reported infecting tomato crops in the country (Barreto et al. 2013). In 2016, about 10% of the plants of the weed Physalis angulata L. were observed with begomovirus-like symptoms (apical chlorosis and stunting) around and within tomato fields in Venda Nova do Imigrante, ES (southeast Brazil). Leaf tissue was collected from 10 symptomatic and five healthy-looking plants, respectively. Total DNA was extracted using the CTAB method in combination with organic solvents (Boiteux et al. 1999). PCR assays with the universal begomovirus primers for DNA-A and DNA-B detection (Rojas et al. 1993) were carried out using total genomic DNA samples (20 ng/μl) as templates. Amplicons of ∼1,100 and ∼550 bp were obtained only in the 10 symptomatic samples, which were in agreement with the expected fragment sizes of DNA-A and DNA-B from begomoviruses (Rojas et al. 1993). These amplicons were directly Sanger sequenced at the Embrapa Vegetables Genomic Analysis Laboratory. BLASTn analyses of the amplicon sequences indicated high nucleotide identities (99 and 98%) with DNA-A and DNA-B of tomato severe rugose virus (ToSRV) isolates (accession nos. JX415196 and MG837739) from Brazil, suggesting the infection of ToSRV in these plants. To fully prove the infection and to characterize the ToSRV isolate associated with P. angulata, the DNA samples were submitted to the rolling circle amplification method followed by nanopore sequencing (Oxford Nanopore Technologies), as previously described (Naito et al. 2019). The full-length DNA-A component of one isolate obtained from P. angulata (MN059848) was 98% identical to the DNA-A genome of a Brazilian ToSRV isolate from tomato (JX415196). The complete DNA-B sequence from the P. angulata isolate (MN059849) was 96% identical to the DNA-B genome of a tomato-infecting ToSRV isolate obtained in southeast Brazil (MG837739). The complete DNA-A component (2,592 nt) displayed all genomic features of the New World species, with one virion-sense open reading frame (ORF) (AV1) and four complementary sense ORFs (AC1, AC2, AC3, and AC4). The complete DNA-B component (2,571 nt) displayed two ORFs: virion sense (BV1) and complementary sense (BC1). The common regions of DNA-A and DNA-B components were identified (183 nt in length), and the presence of iterons was confirmed. ToSRV is so far the most prevalent tomato-infecting begomovirus in Brazil (Fernandes et al. 2008). To our knowledge, this is the first report of natural infection of P. angulata by ToSRV, expanding the viral host range to this widespread tropical and subtropical weed. Considering that P. angulata may function as a relevant natural source of ToSRV inoculum to tomato crops across tropical and subtropical regions, control of the weed should be a part of the integrated management of ToSRV in tomato crops.The author(s) declare no conflict of interest.References:Barreto, S. S., et al. 2013. Phytopathology 103:436. https://doi.org/10.1094/PHYTO-07-12-0174-R Link, ISI, Google ScholarBoiteux, L. S., et al. 1999. J. Am. Soc. Hortic. Sci. 124:32. https://doi.org/10.21273/JASHS.124.1.32 Crossref, ISI, Google ScholarFernandes, F. R., et al. 2008. Virus Genes 36:251. https://doi.org/10.1007/s11262-007-0184-y Crossref, ISI, Google ScholarNaito, F., et al. 2019. Arch. Virol. 164:1907. https://doi.org/10.1007/s00705-019-04254-5 Crossref, ISI, Google ScholarRojas, M. R., et al. 1993. Plant Dis. 77:340. https://doi.org/10.1094/PD-77-0340 Crossref, ISI, Google ScholarThe author(s) declare no conflict of interest.Funding: Funding was provided by Fundação de Apoio à Pesquisa do Distrito Federal (grant no. 193.001.532/2016) and Conselho Nacional de Desenvolvimento Científico e Tecnológico.DetailsFiguresLiterature CitedRelated Vol. 104, No. 2 February 2020SubscribeISSN:0191-2917e-ISSN:1943-7692 DownloadCaptionSymptom of maize ear rot caused by Fusarium sporotrichioides (B. B. Wang et al.). Photo credit: C. X. Duan. Systemic symptoms of alfalfa mosaic virus (AMV) isolate CaM on leaves of potato (X. Z. Nie et al.). Photo credit: X. Z. Nie. Metrics Downloaded 2,533 times Article History Issue Date: 31 Jan 2020Published: 4 Dec 2019First Look: 14 Oct 2019Accepted: 5 Oct 2019 Pages: 600-600 Information© 2020 The American Phytopathological SocietyFundingFundação de Apoio à Pesquisa do Distrito FederalGrant/Award Number: 193.001.532/2016Conselho Nacional de Desenvolvimento Científico e TecnológicoKeywordsBegomoviruspathogen detectionnanopore sequencingThe author(s) declare no conflict of interest.Cited ByCurrent Developments and Challenges in Plant Viral Diagnostics: A Systematic Review5 March 2021 | Viruses, Vol. 13, No. 3Diversity of tomato-infecting begomoviruses and spatiotemporal dynamics of an endemic viral species of the Brazilian Atlantic rain forest biome24 November 2020 | Virus Genes, Vol. 57, No. 1
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