First Report of Phomopsis heveicola (Anamorph of Diaporthe tulliensis ) Causing Leaf Blight of Coffee ( Coffea arabica ) in China
2019; American Phytopathological Society; Volume: 104; Issue: 2 Linguagem: Inglês
10.1094/pdis-09-19-1833-pdn
ISSN1943-7692
AutoresJianhua Gong, Ying Lu, Weihuai Wu, Chun‐ping He, Yue Liang, Xing Huang, Jinlong Zheng, Jingen Xi, Song-Ming Tang, Ke-Xin Yi,
Tópico(s)Forest Insect Ecology and Management
ResumoHomePlant DiseaseVol. 104, No. 2First Report of Phomopsis heveicola (Anamorph of Diaporthe tulliensis) Causing Leaf Blight of Coffee (Coffea arabica) in China PreviousNext DISEASE NOTES OPENOpen Access licenseFirst Report of Phomopsis heveicola (Anamorph of Diaporthe tulliensis) Causing Leaf Blight of Coffee (Coffea arabica) in ChinaJ. L. Gong, Y. Lu, W. H. Wu, C. P. He, Y. Q. Liang, X. Huang, J. L. Zheng, J. G. Xi, S. B. Tang, and K. X. YiJ. L. GongCollege of Plant Protection, Nanjing Agricultural University, Nanjing, Jiangsu 210095, ChinaEnvironment and Plant Protection Institute, Chinese Academy of Tropical Agricultural Sciences/Key Laboratory for Monitoring and Control of Tropical Agricultural and Forest Invasive Alien Pests, Ministry of Agriculture/Hainan Key Laboratory for Detection and Control of Tropical Agricultural Pests, Haikou, Hainan 571101, China, Y. Luhttp://orcid.org/0000-0003-4568-8374Environment and Plant Protection Institute, Chinese Academy of Tropical Agricultural Sciences/Key Laboratory for Monitoring and Control of Tropical Agricultural and Forest Invasive Alien Pests, Ministry of Agriculture/Hainan Key Laboratory for Detection and Control of Tropical Agricultural Pests, Haikou, Hainan 571101, China, W. H. WuEnvironment and Plant Protection Institute, Chinese Academy of Tropical Agricultural Sciences/Key Laboratory for Monitoring and Control of Tropical Agricultural and Forest Invasive Alien Pests, Ministry of Agriculture/Hainan Key Laboratory for Detection and Control of Tropical Agricultural Pests, Haikou, Hainan 571101, China, C. P. HeEnvironment and Plant Protection Institute, Chinese Academy of Tropical Agricultural Sciences/Key Laboratory for Monitoring and Control of Tropical Agricultural and Forest Invasive Alien Pests, Ministry of Agriculture/Hainan Key Laboratory for Detection and Control of Tropical Agricultural Pests, Haikou, Hainan 571101, China, Y. Q. LiangEnvironment and Plant Protection Institute, Chinese Academy of Tropical Agricultural Sciences/Key Laboratory for Monitoring and Control of Tropical Agricultural and Forest Invasive Alien Pests, Ministry of Agriculture/Hainan Key Laboratory for Detection and Control of Tropical Agricultural Pests, Haikou, Hainan 571101, China, X. HuangEnvironment and Plant Protection Institute, Chinese Academy of Tropical Agricultural Sciences/Key Laboratory for Monitoring and Control of Tropical Agricultural and Forest Invasive Alien Pests, Ministry of Agriculture/Hainan Key Laboratory for Detection and Control of Tropical Agricultural Pests, Haikou, Hainan 571101, China, J. L. Zhenghttp://orcid.org/0000-0001-6529-4634Environment and Plant Protection Institute, Chinese Academy of Tropical Agricultural Sciences/Key Laboratory for Monitoring and Control of Tropical Agricultural and Forest Invasive Alien Pests, Ministry of Agriculture/Hainan Key Laboratory for Detection and Control of Tropical Agricultural Pests, Haikou, Hainan 571101, China, J. G. XiEnvironment and Plant Protection Institute, Chinese Academy of Tropical Agricultural Sciences/Key Laboratory for Monitoring and Control of Tropical Agricultural and Forest Invasive Alien Pests, Ministry of Agriculture/Hainan Key Laboratory for Detection and Control of Tropical Agricultural Pests, Haikou, Hainan 571101, China, S. B. TangEnvironment and Plant Protection Institute, Chinese Academy of Tropical Agricultural Sciences/Key Laboratory for Monitoring and Control of Tropical Agricultural and Forest Invasive Alien Pests, Ministry of Agriculture/Hainan Key Laboratory for Detection and Control of Tropical Agricultural Pests, Haikou, Hainan 571101, China, and K. X. Yi†Corresponding author: K. X. Yi; E-mail Address: yikexian@126.comEnvironment and Plant Protection Institute, Chinese Academy of Tropical Agricultural Sciences/Key Laboratory for Monitoring and Control of Tropical Agricultural and Forest Invasive Alien Pests, Ministry of Agriculture/Hainan Key Laboratory for Detection and Control of Tropical Agricultural Pests, Haikou, Hainan 571101, China AffiliationsAuthors and Affiliations J. L. Gong1 2 Y. Lu2 W. H. Wu2 C. P. He2 Y. Q. Liang2 X. Huang2 J. L. Zheng2 J. G. Xi2 S. B. Tang2 K. X. Yi2 † 1College of Plant Protection, Nanjing Agricultural University, Nanjing, Jiangsu 210095, China 2Environment and Plant Protection Institute, Chinese Academy of Tropical Agricultural Sciences/Key Laboratory for Monitoring and Control of Tropical Agricultural and Forest Invasive Alien Pests, Ministry of Agriculture/Hainan Key Laboratory for Detection and Control of Tropical Agricultural Pests, Haikou, Hainan 571101, China Published Online:9 Dec 2019https://doi.org/10.1094/PDIS-09-19-1833-PDNAboutSectionsSupplemental ToolsAdd to favoritesDownload CitationsTrack Citations ShareShare onFacebookTwitterLinked InRedditEmailWechat Coffee (Coffea arabica L.) is a very important crop in China and in the global economy. During March 2019, symptoms of leaf blight were observed on coffee planted at Hainan and Yunnan Provinces in China. Disease incidence ranged from 10 to 20% in the affected coffee plantings, and in extreme cases, 45% out of 200 trees were affected. Symptomatic leaves initially exhibited small, reddish-brown, round or oval spots on the tip of leaves, subsequently expanding in size along the leaf margin. Infected leaves eventually became wilted and dry. Fifty-eight leaves with typical symptoms were randomly collected from five coffee plantations in five counties and then subjected to mycological and pathological analysis. Diseased leaves were cut into small pieces (5 × 5 mm), surface sterilized with 0.1% mercuric chloride solution for 15 s, washed three to four times with sterilized distilled water, and transferred onto potato dextrose agar (PDA) medium in Petri plates. Inoculated Petri plates were incubated at 25°C for 5 days. Representative isolates were transferred to alfalfa extract + Czapek medium. After 7 days of incubation, fungal colonies were cottony white and pale gray on the reverse side. Dark brown globose to subglobose conidiomata (pycnidia) were scattered or in groups and contained cylindrical hyaline conidiophores bearing two types of conidia: alpha- and beta-conidia. The blackish necks of the pycnidia protruded from the surface of the medium. Conidia gathered in yellow tendrils or drop-like conidial cirrus extruding from the ostiole. Alpha-conidia, fusiform, two to three guttulate, 7 to 10 × 2 to 3 μm (n = 200), and beta-conidia, filiform, curved, 12.5 to 19.0 to 25.4 × 1.1 to 1.8 to 2.5 μm (n = 200), similar to Ma et al. (2004). Three single-spore isolates cultured on PDA medium were selected for DNA extraction. The ribosomal internal transcribed spacer (ITS) was amplified by using primers ITS1 and ITS4 (White et al. 1990), translation elongation factor (TEF-1a) by EF-728F and EF-986R (Carbone and Kohn 1999), and β-tubulin gene by Bt2a and Bt2b (Glass and Donaldson 1995). The sequences of ITS (MN393590), TEF-1a (MN399885), and β-tubulin gene (MN399886) were submitted to GenBank. BLAST analysis demonstrated that these sequences were 99% similar to the sequences of ITS (MK429858.1), TEF-1a (MF522196.1), and β-tubulin genes (KR936132.1) published for Phomopsis heveicola (anamorph of Diaporthe tulliensis). Pathogenicity testing was conducted by inoculating 15 healthy coffee plants. Fungal conidia were harvested by flooding 10-day-old single-conidial cultures with sterile water, centrifuging, and adjusting the concentration to 1 × 106 spores/ml. Then, 30-μl conidial suspensions were dropped onto the surfaces of wounded leaves with a sterile needle. Sterile distilled water was used for a control treatment. After inoculation, the plants were kept at 90 to 100% relative humidity at 25 to 28°C in a greenhouse for 2 weeks and monitored daily for lesion development. Seven days after inoculation, all the leaves inoculated presented similar typical symptoms observed under natural conditions, whereas the control leaves showed no symptoms. Koch's postulates were fulfilled by reisolating the fungus and verifying its colony and morphological characters as P. heveicola. This pathogen has been reported on Hevea rubber in China (Jiang et al. 2017). To our knowledge, this is the first report of P. heveicola causing leaf blight of coffee in China.The author(s) declare no conflict of interest.References:Carbone, I., and Kohn, L. M. 1999. Mycologia 91:553. https://doi.org/10.1080/00275514.1999.12061051 Crossref, ISI, Google ScholarGlass, N. L., and Donaldson, G. C. 1995. Appl. Environ. Microbiol. 61:1323. Crossref, ISI, Google ScholarJiang, G. Z., et al. 2017. Trop. Agric. Sci. Tech. 40:10. Google ScholarMa, L., et al. 2004. Mycosystema 23:457. Google ScholarWhite, T. J., et al. 1990. Page 315 in: PCR Protocols: A Guide to Methods and Applications. Academic Press, San Diego, CA. Crossref, Google ScholarJ. L. Gong and Y. Lu contributed equally to this work and should be regarded as co-first authors.The author(s) declare no conflict of interest.Funding: This study was supported by the National Key R&D Program of China (No. 2018YFD0201100).DetailsFiguresLiterature CitedRelated Vol. 104, No. 2 February 2020SubscribeISSN:0191-2917e-ISSN:1943-7692 DownloadCaptionSymptom of maize ear rot caused by Fusarium sporotrichioides (B. B. Wang et al.). Photo credit: C. X. Duan. Systemic symptoms of alfalfa mosaic virus (AMV) isolate CaM on leaves of potato (X. Z. Nie et al.). Photo credit: X. Z. Nie. Metrics Downloaded 3,499 times Article History Issue Date: 31 Jan 2020Published: 9 Dec 2019First Look: 15 Oct 2019Accepted: 13 Oct 2019 Page: 570 Information© 2020 The American Phytopathological SocietyFundingNational Key R&D Program of ChinaGrant/Award Number: No. 2018YFD0201100Keywordsfungitropical plantsfield cropspathogen detectionThe author(s) declare no conflict of interest.Cited ByMolecular Phylogenetic Diversity and Biological Characterization of Diaporthe Species Associated with Leaf Spots of Camellia sinensis in Taiwan14 July 2021 | Plants, Vol. 10, No. 7Specific PCR‐based detection of Phomopsis heveicola the cause of leaf blight of Coffee ( Coffea arabica L.) in China18 December 2020 | Letters in Applied Microbiology, Vol. 72, No. 4Bioactive Secondary Metabolites of the Genus Diaporthe and Anamorph Phomopsis from Terrestrial and Marine Habitats and Endophytes: 2010–201921 January 2021 | Microorganisms, Vol. 9, No. 2Epigenetic Modifiers Affect the Bioactive Compounds Secreted by an Endophyte of the Tropical Plant Piper longum23 December 2020 | Molecules, Vol. 26, No. 1
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