First Report of White Thread Blight Caused by Ceratobasidium niltonsouzanum on Yerba Mate in Argentina
2019; American Phytopathological Society; Volume: 104; Issue: 2 Linguagem: Inglês
10.1094/pdis-08-19-1603-pdn
ISSN1943-7692
AutoresNelson Bernardi Lima, Mario Kryvenki, Cinthia Conforto, Dannae Lilia Serri, R. Krämer, Mónica Esther María Roca, Silvina Vargas Gil,
Tópico(s)Yeasts and Rust Fungi Studies
ResumoHomePlant DiseaseVol. 104, No. 2First Report of White Thread Blight Caused by Ceratobasidium niltonsouzanum on Yerba Mate in Argentina PreviousNext DISEASE NOTES OPENOpen Access licenseFirst Report of White Thread Blight Caused by Ceratobasidium niltonsouzanum on Yerba Mate in ArgentinaN. B. Lima, M. A. Kryvenki, C. Conforto, D. Serri, R. Kramer, M. Roca, and S. Vargas-GilN. B. Lima†Corresponding author: N. B. Lima; E-mail Address: nblima@hotmail.comhttp://orcid.org/0000-0002-6145-5119Instituto de Patología Vegetal (IPAVE), Centro de Investigaciones Agropecuarias (CIAP), Instituto Nacional de Tecnología Agropecuaria (INTA), Unidad de Fitopatología y Modelización Agrícola (UFYMA), CONICET, Córdoba, C.P. X5020, Argentina, M. A. KryvenkiEstación Experimental Agropecuaria Cerro Azul, INTA, Cerro Azul, Misiones, C.P. 3313, Argentina, C. ConfortoInstituto de Patología Vegetal (IPAVE), Centro de Investigaciones Agropecuarias (CIAP), Instituto Nacional de Tecnología Agropecuaria (INTA), Unidad de Fitopatología y Modelización Agrícola (UFYMA), CONICET, Córdoba, C.P. X5020, Argentina, D. SerriInstituto de Patología Vegetal (IPAVE), Centro de Investigaciones Agropecuarias (CIAP), Instituto Nacional de Tecnología Agropecuaria (INTA), Unidad de Fitopatología y Modelización Agrícola (UFYMA), CONICET, Córdoba, C.P. X5020, Argentina, R. KramerOIT Andresito, Estación Experimental Agropecuaria Montecarlo, Misiones, C.P. 3313, Argentina, M. Rocahttp://orcid.org/0000-0002-9333-8865Servicio Nacional de Sanidad y Calidad Agroalimentaria–Universidad Nacional La Rioja, C.P. 5300, La Rioja, Argentina, and S. Vargas-GilInstituto de Patología Vegetal (IPAVE), Centro de Investigaciones Agropecuarias (CIAP), Instituto Nacional de Tecnología Agropecuaria (INTA), Unidad de Fitopatología y Modelización Agrícola (UFYMA), CONICET, Córdoba, C.P. X5020, Argentina AffiliationsAuthors and Affiliations N. B. Lima1 † M. A. Kryvenki2 C. Conforto1 D. Serri1 R. Kramer3 M. Roca4 S. Vargas-Gil1 1Instituto de Patología Vegetal (IPAVE), Centro de Investigaciones Agropecuarias (CIAP), Instituto Nacional de Tecnología Agropecuaria (INTA), Unidad de Fitopatología y Modelización Agrícola (UFYMA), CONICET, Córdoba, C.P. X5020, Argentina 2Estación Experimental Agropecuaria Cerro Azul, INTA, Cerro Azul, Misiones, C.P. 3313, Argentina 3OIT Andresito, Estación Experimental Agropecuaria Montecarlo, Misiones, C.P. 3313, Argentina 4Servicio Nacional de Sanidad y Calidad Agroalimentaria–Universidad Nacional La Rioja, C.P. 5300, La Rioja, Argentina Published Online:18 Dec 2019https://doi.org/10.1094/PDIS-08-19-1603-PDNAboutSectionsSupplemental ToolsAdd to favoritesDownload CitationsTrack Citations ShareShare onFacebookTwitterLinked InRedditEmailWechat Yerba mate (Ilex paraguariensis A.St.-Hil.) is a native species of the subtropical and temperate regions of South America. In the Misiones province, in northeastern Argentina, yerba mate is an important agricultural crop grown on approximately 150,000 ha. In March 2019, diseased leaves and stems from four fields near Comandante Andresito city were received for diagnosis at the Instituto de Patología Vegetal (IPAVE) and Instituto Nacional de Tecnología Agropecuaria, Argentina. Disease symptoms were accompanied by white mycelium threads in the young branches. The threads extended longitudinally, along the surface of the stem, which later developed into leaf blight, with leaves hanging on white threads of fungal hyphae. Symptoms were the same in samples from all four fields (100% prevalence), and disease incidence varied between 50 and 80%. Isolations were performed by transferring small sections of diseased plant tissue, disinfested in 1.5% sodium hypochlorite for 1 min, washed with sterile distilled water, transferred to Petri dishes containing potato dextrose agar (PDA), and incubated at 25°C in the dark. Over 10 isolations from five plants, eight Rhizoctonia-like colonies were isolated. Pure cultures were obtained by transferring hyphal tips to plates containing PDA. Four isolates (IPAVE 0075, 0076, 0077, and 0078) were selected as representatives for characterization and deposited at the Culture Collection of IPAVE (Córdoba, Argentina). On PDA, the colonies initially had white aerial mycelia, and concentric rings were visible on the reverse side. The colonies turned brown after approximately 30 days. Hyphae were branched with 90° angulation, a typical character of members of the form genus Rhizoctonia. For molecular identification, the internal transcribed spacer region was amplified by polymerase chain reaction (White et al. 1990) and sequenced. Phylogenetic analyses using Bayesian inference grouped the isolates from yerba mate with the type species of Ceratobasidium niltonsouzanum (Melo et al. 2018) with a high posterior probability (0.98). Pathogenicity testing was conducted using toothpicks colonized with the pathogen (Souza et al. 2009). Inoculum was produced on PDA medium by allowing the isolates to colonize sterilized toothpicks placed on PDA and incubating for 7 days at 25°C under a 12-h photoperiod. Yerba mate plants (24 months old) were inoculated by inserting the colonized toothpicks into the base of the leaf petiole. Plants were kept in the greenhouse at 25°C under high humidity for 30 days and observed daily (Souza et al. 2009). Four control plants were mock inoculated using only sterilized toothpicks. White thread blight symptoms were observed on all inoculated plants after 20 days, whereas control plants remained symptomless. C. niltonsouzanum was reisolated from symptomatic tissue (leaves and petiole) of the inoculated plants, with a frequency of 100%, thereby validating Koch's postulates. The experiment was performed twice. C. niltonsouzanum has been reported as a pathogen on numerous crops including Coffea arabica, Camellia sinensis, Azadirachta indica, and Eugenia uniflora in Brazil (de Melo et al. 2018). This work improves knowledge surrounding the etiology of the disease associated with yerba mate in Argentina, which is essential information for the identification of appropriate management practices. To our knowledge, this is the first report of C. niltonsouzanum causing white thread blight of yerba mate in Argentina and worldwide.The author(s) declare no conflict of interest.References:de Melo, M. P., et al. 2018. Trop. Plant Pathol. 43:559. https://doi.org/10.1007/s40858-018-0237-x Crossref, ISI, Google ScholarSouza, E. C., et al. 2009. Summa Phytopathol. 35:9. https://doi.org/10.1590/S0100-54052009000100001 Crossref, Google ScholarWhite, T. J., et al. 1990. Page 315 in: PCR Protocols: A Guide to Methods and Applications. Academic Press, San Diego, CA. Crossref, Google ScholarN. B. Lima and M. A. Kryvenki contributed equally to this work.The author(s) declare no conflict of interest.Funding: Funding was provided by Centro Regional Misiones – INTA, CONICET (PIP N° 11220150100061CO) and INTA (INTA PD I090).DetailsFiguresLiterature CitedRelated Vol. 104, No. 2 February 2020SubscribeISSN:0191-2917e-ISSN:1943-7692 DownloadCaptionSymptom of maize ear rot caused by Fusarium sporotrichioides (B. B. Wang et al.). Photo credit: C. X. Duan. Systemic symptoms of alfalfa mosaic virus (AMV) isolate CaM on leaves of potato (X. Z. Nie et al.). Photo credit: X. Z. Nie. Metrics Downloaded 3,315 times Article History Issue Date: 31 Jan 2020Published: 18 Dec 2019First Look: 14 Oct 2019Accepted: 10 Oct 2019 Pages: 572-572 Information© 2020 The American Phytopathological SocietyFundingCONICETGrant/Award Number: PIP N° 11220150100061COINTAGrant/Award Number: INTA PD I090Keywordspathogen detectionyerba mateRhizoctonia-like fungiThe author(s) declare no conflict of interest.Cited ByReinstating Ceratobasidium lantanae-camarae: the white thread blight fungus on the pantropical weed Lantana camara14 April 2021 | Australasian Plant Pathology, Vol. 99
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