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BIBTEX RIS

Quantification of bisphenol A in urine samples from children studying in public schools from the Brazilian Capital

2019; Elsevier BV; Volume: 152; Linguagem: Inglês

10.1016/j.microc.2019.104347

1095-9149

Hadassa S. R. P. Moura, Priscilla Roberta Silva Rocha, Angélica Amorim Amato, Fernando F. Sodré,

Toxic Organic Pollutants Impact

Human exposure to endocrine disrupting chemicals (EDCs) is broadly investigated due to possible adverse effects to human health. Bisphenol A (BPA) is a chemical of great concern due to its wide use in industrial products including canned food. Exposure during childhood is of special concern because the developing endocrine system is particularly sensitive to EDC. Therefore, there is an urgent need to asses degree of exposure and possible adverse effects. In this study, we validated a liquid-liquid microextraction method coupled to liquid chromatography (gradient elution)/tandem high-resolution mass spectrometry (HR-MRM acquisition) to assess BPA (free + deconjugated) in 343 urine samples from children attending public schools in Ceilandia (Federal District, Brazil). For quantification, matrix-matched calibration with internal standardization were used under a linear working range from 1.0 to 2000 ng/mL (R² = 0.998). Limits of quantification and detection of the method were 0.1 and 0.03 ng/mL, respectively. Matrix effect (96 ± 12%) was neglected by the use of deuterated BPA as surrogate, while minimal losses were depicted during the extraction (recovery of 86 ± 14%). Inter- and intra-day precision were below 11 and 12%, respectively. BPA was found in 89.5% of the investigated samples, with concentrations varying from 0.11 to 123 ng/mL. Average BPA (3.88 ng/L) was slightly higher than previous reports investigating Brazilian children, and close to data produced in countries with similar socio-economic indicators, such as India and China.