Portal de Periódicos da CAPES

First Report of Pectobacterium aroidearum Causing Soft Rot of Amorphophallus konjac in China

2019; American Phytopathological Society; Volume: 104; Issue: 3 Linguagem: Inglês

10.1094/pdis-09-19-1960-pdn

1943-7692

Huanyu Wei, Min Yang, Wei-hua Pei, Wei Wei, Feiyan Huang, Jiani Liu, Yu Zhong, Wenting Hu, Ying Dong, Hairu Chen, Lei Yu,

Irrigation Practices and Water Management

HomePlant DiseaseVol. 104, No. 3First Report of Pectobacterium aroidearum Causing Soft Rot of Amorphophallus konjac in China PreviousNext DISEASE NOTES OPENOpen Access licenseFirst Report of Pectobacterium aroidearum Causing Soft Rot of Amorphophallus konjac in ChinaHuan-yu Wei, Min Yang, Wei-hua Pei, Wei Wei, Fei-yan Huang, Jia-ni Liu, Yu Zhong, Wen-ting Hu, Dong Ying, Hai-ru Chen, and Lei YuHuan-yu WeiCollege of Agronomy/Yunnan Urban Agricultural Engineering and Technological Research Center, Kunming University, Yunnan Kunming 650214, ChinaCollege of Plant Protection, Yunnan Agricultural University, Yunnan Kunming 650201, China, Min Yanghttp://orcid.org/0000-0003-3093-3576College of Agronomy/Yunnan Urban Agricultural Engineering and Technological Research Center, Kunming University, Yunnan Kunming 650214, China, Wei-hua Peihttp://orcid.org/0000-0002-6543-1157Agricultural Environment and Resource Research Institute, Yunnan Academy of Agricultural Sciences, Kunming 650205, China, Wei WeiCollege of Agronomy/Yunnan Urban Agricultural Engineering and Technological Research Center, Kunming University, Yunnan Kunming 650214, China, Fei-yan Huanghttp://orcid.org/0000-0002-5166-0848College of Agronomy/Yunnan Urban Agricultural Engineering and Technological Research Center, Kunming University, Yunnan Kunming 650214, China, Jia-ni LiuCollege of Agronomy/Yunnan Urban Agricultural Engineering and Technological Research Center, Kunming University, Yunnan Kunming 650214, China, Yu ZhongCollege of Agronomy/Yunnan Urban Agricultural Engineering and Technological Research Center, Kunming University, Yunnan Kunming 650214, China, Wen-ting HuCollege of Agronomy/Yunnan Urban Agricultural Engineering and Technological Research Center, Kunming University, Yunnan Kunming 650214, China, Dong YingDongying Agriculture and Rural Affairs Bureau, Dongying 257091, China, Hai-ru ChenCollege of Plant Protection, Yunnan Agricultural University, Yunnan Kunming 650201, China, and Lei Yu†Corresponding author: L. Yu; E-mail Address: yulei0425@163.comCollege of Agronomy/Yunnan Urban Agricultural Engineering and Technological Research Center, Kunming University, Yunnan Kunming 650214, China AffiliationsAuthors and Affiliations Huan-yu Wei1 2 Min Yang1 Wei-hua Pei3 Wei Wei1 Fei-yan Huang1 Jia-ni Liu1 Yu Zhong1 Wen-ting Hu1 Dong Ying4 Hai-ru Chen2 Lei Yu1 † 1College of Agronomy/Yunnan Urban Agricultural Engineering and Technological Research Center, Kunming University, Yunnan Kunming 650214, China 2College of Plant Protection, Yunnan Agricultural University, Yunnan Kunming 650201, China 3Agricultural Environment and Resource Research Institute, Yunnan Academy of Agricultural Sciences, Kunming 650205, China 4Dongying Agriculture and Rural Affairs Bureau, Dongying 257091, China Published Online:30 Dec 2019https://doi.org/10.1094/PDIS-09-19-1960-PDNAboutSectionsSupplemental ToolsAdd to favoritesDownload CitationsTrack Citations ShareShare onFacebookTwitterLinked InRedditEmailWechat Konjac (Amorphophallus konjac) is an important cash crop in southwest China, grown as a source of glucomannan for industrial use. In July 2019, symptoms of stem soft rot were observed on A. konjac in Kunming (24.97697941°N, 102.79377675°E), Yunnan province (southwestern China). Symptoms included stem soft rot with a foul smell, inner part of plant disintegration, and wilting of leaves. Twenty-five to 30% of the plants in the field were damaged. Bacteria were isolated on LB (tryptone/yeast extract/NaCl) agar medium and incubated at 28 to 30°C for 48 to 78 h. The isolated colonies were streaked on LB medium for plates to obtain single colonies. The morphology of the bacterium was short, rod-shaped, with flagellum, with a slightly obtuse circle at both ends. DNA of bacterial isolates was extracted, and polymerase chain reaction (PCR) amplifications were performed using 16S rDNA primers (27F, 5′-AGAGTTTGATCCTGGCTCAG-3′; 1492R, 5′-GGTTACCTTGTTACGACTT-3′) and gapA primers (gapA-7-F and gapA-938-R) (Cigna et al. 2017) followed by sequencing. The 16S rDNA sequence of the 1,442-bp product (strain Ecc-K1, accession no. MN161137) had 100% similarity to the 16S rDNA sequences of Pectobacterium aroidearum (MK875007 and NR159926) by using BLAST (Altschul et al. 1990). Phylogenetic analysis of 16S rDNA performed by the CLUSTAL Omega tool (Sievers et al. 2011) showed that the new sequence (accession no. MN161137) and the P. aroidearum NR159926 type strain sequences comprise the same cluster. BLAST analysis (NCBI) repeated with the sequence MN161137 showed the most similar accession P. aroidearum strain Ecc15b MK961266.1 (99.93% similarity) and grouped MN161137 with Ec15b and Pcc M1 DQ205331.1 distant from other P. aroidearum. The P. aroidearum accessions MK875007 and NR159926 had 99.86 and 99.99.65% similarity to MN161137, respectively, and grouped with other P. aroidearum. Similarly, the gapA sequence of the 870-bp product (accession no. MN478003) had 95.84% similarity to the gapA sequences of CP024956 by using BLAST. Moreover, biochemical reactions using the Biolog GEN III System (Biolog, Haywood, CA) identified that the new isolated strain has a similarity value between the strain and P. aroidearum of 0.660 (24 h), greater than the critical value of 0.50, and the genetic distance was 5.389; the identification result has the highest similarity index to P. aroidearum. Therefore, combined results from morphological, molecular, and biochemical reaction results confirmed this species as P. aroidearum (Nabhan et al. 2013). Pathogenicity tests were confirmed on 1-year-old A. konjac seedlings was injected into stem and petiole with a bacterial suspension containing 108 CFU/ml (10 μl/inoculation site). Control seedlings received sterile water. Inoculated and control seedlings (six each) were kept in a greenhouse and watered as needed. Stem soft rot, inner medulla disintegration, and wilt of leaves symptoms developed on inoculated seedlings within 3 to 10 days. The bacterium was reisolated from inoculated seedlings and was identified by above PCR and DNA sequencing methods, thus fulfilling Koch's postulates. No symptoms were visible in the control seedlings. P. aroidearum has been reported as a pathogen of Chinese cabbage (Xie et al. 2018) and Cucurbita pepo (Moraes et al. 2017). To our knowledge, this is the first report of soft rot caused by P. aroidearum on A. konjac in China, which expanded the known host range for this pathogen.The author(s) declare no conflict of interest.References:Altschul, S. F., et al. 1990. J. Mol. Biol. 215:403. https://doi.org/10.1016/S0022-2836(05)80360-2 Crossref, ISI, Google ScholarCigna, J., et al. 2017. Plant Dis. 101:1278. https://doi.org/10.1094/PDIS-12-16-1810-RE Link, ISI, Google ScholarMoraes, A. J. G., et al. 2017. Plant Dis. 101:379. https://doi.org/10.1094/PDIS-08-16-1168-PDN Link, ISI, Google ScholarNabhan, S., et al. 2013. Int. J. Syst. Evol. Microbiol. 63:2520. https://doi.org/10.1099/ijs.0.046011-0 Crossref, ISI, Google ScholarSievers, F., et al. 2011. Mol. Syst. Biol. 7:539. https://doi.org/10.1038/msb.2011.75 Crossref, ISI, Google ScholarXie, H., et al. 2018. Plant Dis. 102:674. https://doi.org/10.1094/PDIS-07-17-1059-PDN Link, ISI, Google ScholarThe author(s) declare no conflict of interest.Funding: This research was supported by the National Natural Science Foundation of China (No. 31660426), Yunnan Provincial Department of Science and Technology (Nos. 2017FH001-005 and 2018HB100), and project of the Key Disciplines (Ecology) Project of Yunnan Education Department (No. 2018JS389).DetailsFiguresLiterature CitedRelated Vol. 104, No. 3 March 2020SubscribeISSN:0191-2917e-ISSN:1943-7692 DownloadCaptionPathogenicity of Lasiodiploidia pseudotheobromae in a coffee plant 3 days after inoculation (R. L. Freitas-Lopes et al.). Photo credit: U. P. Lopes. Seedling blight of soybean caused by soilborne pathogens (J. R. Lamichhane et al.). Photo credit: M. I. Chilvers. Metrics Downloaded 3,287 times Article History Issue Date: 3 Mar 2020Published: 30 Dec 2019First Look: 23 Oct 2019Accepted: 21 Oct 2019 Page: 969 Information© 2020 The American Phytopathological SocietyFundingNational Natural Science Foundation of ChinaGrant/Award Number: 31660426Yunnan Provincial Department of Science and TechnologyGrant/Award Number: 2017FH001-005Grant/Award Number: 2018HB100Key Disciplines (Ecology) Project of Yunnan Education DepartmentGrant/Award Number: 2018JS389KeywordsPectobacterium aroidearumAmorphophallus konjacThe author(s) declare no conflict of interest.Cited ByComparative physiological and transcriptomic profiles reveal regulatory mechanisms of soft rot disease resistance in Amorphophallus spp.Physiological and Molecular Plant Pathology, Vol. 118Effects of Different Pesticide Treatments on Soft Rot Control and Yield of KonjacIOP Conference Series: Earth and Environmental Science, Vol. 687, No. 1