Produção Nacional
Revisado por pares
Trypanosoma rangeli 28Sβ Ribosomal Gene Allows Intra and Interspecific Molecular Differentiation
2019; Mary Ann Liebert, Inc.; Volume: 20; Issue: 2 Linguagem: Inglês
10.1089/vbz.2019.2496
ISSN1557-7759
AutoresRenato Elias Rodrigues de Souza Santos, Lucila Langoni Naves, Emanuella Francisco Fajardo, Luı́s Eduardo Ramı́rez, Eliane Lages-Silva, André Luiz Pedrosa, Keila Adriana Magalhães Ferreira,
Tópico(s)Synthesis and Biological Evaluation
ResumoTrypanosoma rangeli is an avirulent flagellate protozoan that could mislead correct diagnosis of Trypanosoma cruzi infection, the causative agent of Chagas' disease, given their high similarity. Besides, T. rangeli presents two genetic groups, whose differentiation is achieved mainly by molecular approaches. In this context, ribosomal DNA (rDNA) is a useful target for intra and interspecific molecular differentiation. Analyzing the rDNA of T. rangeli and comparison with other trypanosomatid species, two highly divergent regions (Trβ1 and Trβ2) within the 28Sβ gene were found. Those regions were amplified and sequenced in KP1(+) and KP1(−) strains of T. rangeli, revealing group-specific polymorphisms useful for intraspecific distinction through restriction fragment length polymorphism technique. Also, amplification of Trβ1 allowed differentiation between T. rangeli and T. cruzi. Trβ2 predicted restriction length profile, allowed differentiation between T. rangeli, T. cruzi, Trypanosoma brucei, and Leishmania braziliensis, increasing the use of Trβ1 and Trβ2 beyond a molecular approach for T. rangeli genotyping, but also as a useful target for trypanosomatid classification.
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