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First Report of Grapevine ( Vitis sp.) Cluster Blight Caused by Fusarium proliferatum in Russia

2019; American Phytopathological Society; Volume: 104; Issue: 3 Linguagem: Inglês

10.1094/pdis-05-19-0938-pdn

1943-7692

E.G. Yurchenko, N. V. Savchuk, Е. В. Поротикова, С. В. Виноградова,

Horticultural and Viticultural Research

HomePlant DiseaseVol. 104, No. 3First Report of Grapevine (Vitis sp.) Cluster Blight Caused by Fusarium proliferatum in Russia PreviousNext DISEASE NOTES OPENOpen Access licenseFirst Report of Grapevine (Vitis sp.) Cluster Blight Caused by Fusarium proliferatum in RussiaE. G. Yurchenko, N. V. Savchuk, E. V. Porotikova, and S. V. VinogradovaE. G. YurchenkoNorth Caucasian Regional Research Institute of Horticulture and Viticulture, Krasnodar, 350072, Russia, N. V. SavchukNorth Caucasian Regional Research Institute of Horticulture and Viticulture, Krasnodar, 350072, Russia, E. V. Porotikova†Corresponding author: E. V. Porotikova; E-mail Address: plantvirus@mail.ruhttp://orcid.org/0000-0003-1063-0308Research Center of Biotechnology, Russian Academy of Sciences, Moscow, 119071, Russia, and S. V. VinogradovaResearch Center of Biotechnology, Russian Academy of Sciences, Moscow, 119071, Russia AffiliationsAuthors and Affiliations E. G. Yurchenko1 N. V. Savchuk1 E. V. Porotikova2 † S. V. Vinogradova2 1North Caucasian Regional Research Institute of Horticulture and Viticulture, Krasnodar, 350072, Russia 2Research Center of Biotechnology, Russian Academy of Sciences, Moscow, 119071, Russia Published Online:30 Dec 2019https://doi.org/10.1094/PDIS-05-19-0938-PDNAboutSectionsSupplemental ToolsAdd to favoritesDownload CitationsTrack Citations ShareShare onFacebookTwitterLinked InRedditEmailWechat Viticulture is an important economic sector in Russia, with 91,500 ha producing 580,000 tons in 2017 (according to Statistics Service of the Russian Federation, https://gks.ru/enterprise_economy). The monitoring of table "Euro-American" hybrid varieties Citron, Moldova, Augustin, and Ubiley Novocherkasska was conducted in a 50-ha vineyard in Krasnodar region in 2017. Blight of the peduncles and rachis was first observed during the flowering and later on berries of nearly 25 to 30% of each hybrid variety. The infected parts of the rachis were washed for 2 min under running water followed by 2% NaClO for 30 s and rinsed with sterilized water. Small pieces were separated and placed on potato dextrose agar (PDA). Colonies with abundant aerial mycelium were observed in 5 days. A hyphal tip was taken, put on PDA, and incubated for 7 to 10 days. The colonies of mycelium were originally white and then pink to pinkish-purple. Violet pigments diffused into the agar after 7 days of incubation at 25°C. Five single cultures were consistently isolated. Based on cultural and conidial morphology, isolates were identified as Fusarium proliferatum (Matsushima) Nirenberg (Leslie and Summerell 2006). On average through five isolates, macroconidia were slender, thin-walled, three to five septate, 20.7 to 45.5 × 2.8 to 6.1 μm (n = 50), with curved apical cells and poorly developed basal cells. Microconidia were thin-walled, hyaline, club-shaped, 4.5 to 10.8 × 1.5 to 3.2 μm (n = 50), and formed in chains and in false heads from monophialides and polyphialides. Chlamydospores were absent. For molecular identification, the internal transcribed spacer region (ITS), beta-tubulin (β-tub), translation elongation factor 1α (EF1α), and RNA polymerase II genes (RPB2) of one isolate were amplified, sequenced using primers ITS1/ITS4 (White et al. 1990), T1/T22 (O'Donnell and Cigelnik 1997), EF1f/EF2r, and 5f2/7cr and 7cf/11ar (O'Donnell et al. 2010) and deposited at GenBank (MK598060, MK598059, MK598058, and MK598061, respectively). BLAST analysis showed that the ITS, β-tub, EF1α, and RPB2 sequences had 100% identity to F. proliferatum (MK158221.1, LT841257.1, MH178093.1, and LT841252.1, respectively). In addition, the sequences of TUB and RPB2 showed 99.8 and 99.44% identities to the sequences of the Fusarium fujikuroi Nirenberg species complex (FD_01776 and FD_03678) in the Fusarium-ID database (Geiser et al. 2004). EF1α and ITS showed 99.84 and 97% identities to the sequences of F. proliferatum and F. concolor, respectively (FD_01389 and FD_01847). Multilocus sequence typing (MLST) using four genes in Fusarium MLST database showed 99.74% identity with F. fujikuroi (NRRL 13308). The pathogenicity tests of five isolates were conducted on clusters and young green shoots of the most common in Russia table hybrid varieties Moldova (late ripening red grape) and Augustin (early ripening white grape) in a single experiment. Fifteen shoots of each hybrid variety were inoculated with 50 μl of conidial suspension (1 × 106 conidia/ml) of each isolate and were maintained at 25°C and 70% relative humidity. Tissue necrosis of inoculated shoots and blight of clusters were observed in 4 days after inoculation. In 2 weeks, complete tissue blight was observed, similar to that observed in the field, whereas the control shoots treated with water remained asymptomatic. The pathogen was reisolated from the inoculated shoots, thus completing Koch's postulates. Previously, F. proliferatum was identified as the pathogen causing fruit rot on grapevine in Pakistan and China (Ghuffar et al. 2018; Wang et al. 2015). Here we describe different symptoms caused by F. proliferatum on grapevine. The results of this study have great importance for improving phytosanitary monitoring and integrated disease control of vineyards, because F. proliferatum can seriously limit the production of table grapes in the Krasnodar region. To our knowledge, this is the first report of grapevine cluster blight caused by F. proliferatum in Russia.The author(s) declare no conflict of interest.References:Geiser, D. M., et al. 2004. Eur. J. Plant Pathol. 110:473. https://doi.org/10.1023/B:EJPP.0000032386.75915.a0 Crossref, ISI, Google ScholarGhuffar, S., et al. 2018. Int. J. Phytopathol. 7:85. https://doi.org/10.33687/phytopath.007.02.2589 Crossref, Google ScholarLeslie, J. F., and Summerell, B. A. 2006. The Fusarium Laboratory Manual. Blackwell, Ames, IA. https://doi.org/10.1002/9780470278376 Crossref, Google ScholarO'Donnell, K., and Cigelnik, E. 1997. Mol. Phylogenet. Evol. 7:103. https://doi.org/10.1006/mpev.1996.0376 Crossref, ISI, Google ScholarO'Donnell, K., et al. 2010. J. Clin. Microbiol. 48:3708. https://doi.org/10.1128/JCM.00989-10 Crossref, ISI, Google ScholarWang, Y., et al. 2015. Plant Dis. 99:1180. https://doi.org/10.1094/PDIS-12-14-1351-PDN Link, ISI, Google ScholarWhite, T. J., et al. 1990. Page 315 in: PCR Protocols: A Guide to Methods and Applications. Academic Press, San Diego, CA. Crossref, Google ScholarThe author(s) declare no conflict of interest.Funding: Funding was provided by Russian Science Foundation (grant no. 17-76-10067). Sequencing was performed using the experimental climate control facility U-73547 and partially funded by the Ministry of Education and Science of Russian Federation.DetailsFiguresLiterature CitedRelated Vol. 104, No. 3 March 2020SubscribeISSN:0191-2917e-ISSN:1943-7692 DownloadCaptionPathogenicity of Lasiodiploidia pseudotheobromae in a coffee plant 3 days after inoculation (R. L. Freitas-Lopes et al.). Photo credit: U. P. Lopes. Seedling blight of soybean caused by soilborne pathogens (J. R. Lamichhane et al.). Photo credit: M. I. Chilvers. Metrics Downloaded 2,629 times Article History Issue Date: 3 Mar 2020Published: 30 Dec 2019First Look: 30 Oct 2019Accepted: 23 Oct 2019 Pages: 991-991 Information© 2020 The American Phytopathological SocietyFundingRussian Science FoundationGrant/Award Number: 17-76-10067KeywordsgrapevinevineyardFusarium proliferatumThe author(s) declare no conflict of interest.Cited ByTo the study of saprotrophic micoromycetes complexes associated with wild and cultivated vines of grapes in the Western Ciscaucasia (Russia)IOP Conference Series: Earth and Environmental Science, Vol. 848, No. 1BIO Web of Conferences, Vol. 21