First Report of Cotton leafroll dwarf virus Infecting Upland Cotton ( Gossypium hirsutum ) in Texas
2019; American Phytopathological Society; Volume: 104; Issue: 3 Linguagem: Inglês
10.1094/pdis-09-19-2008-pdn
ISSN1943-7692
AutoresOlufemi J. Alabi, Thomas Isakeit, Robert N. Vaughn, David M. Stelly, Kassie Conner, Brianna C. Gaytán, Cecilia Villegas, Christian Hitzelberger, Luis M. De Santiago, Cecilia Monclova-Santana, Judith K. Brown,
Tópico(s)Research in Cotton Cultivation
ResumoHomePlant DiseaseVol. 104, No. 3First Report of Cotton leafroll dwarf virus Infecting Upland Cotton (Gossypium hirsutum) in Texas PreviousNext DISEASE NOTES OPENOpen Access licenseFirst Report of Cotton leafroll dwarf virus Infecting Upland Cotton (Gossypium hirsutum) in TexasOlufemi J. Alabi, Thomas Isakeit, Robert Vaughn, David Stelly, Kassie N. Conner, Brianna C. Gaytán, Cecilia Villegas, Christian Hitzelberger, Luis De Santiago, Cecilia Monclova-Santana, and Judith K. BrownOlufemi J. Alabi†Corresponding author: O. J. Alabi; E-mail Address: alabi@tamu.eduhttp://orcid.org/0000-0002-2471-7052Department of Plant Pathology and Microbiology, Texas A&M AgriLife Research and Extension Center, Weslaco, TX 78596, Thomas IsakeitDepartment of Plant Pathology and Microbiology, Texas A&M University, College Station, TX 77843, Robert VaughnDepartment of Soil and Crop Sciences, Texas A&M University, College Station, TX 77843, David StellyDepartment of Soil and Crop Sciences, Texas A&M University, College Station, TX 77843, Kassie N. ConnerAlabama Cooperative Extension System, Auburn University, Auburn, AL 36849, Brianna C. GaytánDepartment of Plant Pathology and Microbiology, Texas A&M AgriLife Research and Extension Center, Weslaco, TX 78596, Cecilia VillegasDepartment of Plant Pathology and Microbiology, Texas A&M AgriLife Research and Extension Center, Weslaco, TX 78596, Christian HitzelbergerDepartment of Soil and Crop Sciences, Texas A&M University, College Station, TX 77843, Luis De SantiagoDepartment of Soil and Crop Sciences, Texas A&M University, College Station, TX 77843, Cecilia Monclova-SantanaDepartment of Plant Pathology and Microbiology, Texas A&M AgriLife Research and Extension Center, Lubbock, TX 79403, and Judith K. BrownSchool of Plant Sciences, The University of Arizona, Tucson, AZ 85721 AffiliationsAuthors and Affiliations Olufemi J. Alabi1 † Thomas Isakeit2 Robert Vaughn3 David Stelly3 Kassie N. Conner4 Brianna C. Gaytán1 Cecilia Villegas1 Christian Hitzelberger3 Luis De Santiago3 Cecilia Monclova-Santana5 Judith K. Brown6 1Department of Plant Pathology and Microbiology, Texas A&M AgriLife Research and Extension Center, Weslaco, TX 78596 2Department of Plant Pathology and Microbiology, Texas A&M University, College Station, TX 77843 3Department of Soil and Crop Sciences, Texas A&M University, College Station, TX 77843 4Alabama Cooperative Extension System, Auburn University, Auburn, AL 36849 5Department of Plant Pathology and Microbiology, Texas A&M AgriLife Research and Extension Center, Lubbock, TX 79403 6School of Plant Sciences, The University of Arizona, Tucson, AZ 85721 Published Online:5 Jan 2020https://doi.org/10.1094/PDIS-09-19-2008-PDNAboutSections ToolsAdd to favoritesDownload CitationsTrack Citations ShareShare onFacebookTwitterLinked InRedditEmailWechat Virus-like symptoms were observed on two upland cotton (Gossypium hirsutum L.) research plots in Brazos County, Texas, during July 2019, and disease incidence ranged between 5 and 20% across fields. Symptoms consisted of leaf distortion, upward leaf cupping, leaf midrib and vein reddening, shortened internodes, and dwarfing of affected plants, which were reminiscent of those attributed to cotton leafroll dwarf virus (CLRDV, genus Polerovirus, family Luteoviridae). Total RNA was isolated from 20 field-collected samples (symptomatic = 15, asymptomatic = 5) using the Spectrum Plant Total RNA Kit (Sigma-Aldrich, St. Louis, MO), followed by cDNA synthesis (PrimeScript First Strand cDNA Synthesis Kit, Takara Bio USA, Mountain View, CA), according to the manufacturers' instructions. PCR amplification was carried out with primers CLRDV3675F/Pol3982R that target a 310-bp partial ORF3-5 fragment (Sharman et al. 2015), AL674F/Al1407R to amplify a 733-bp product of the partial P0/P1 (Avelar et al. 2019), and P0_51F/P0_916R to amplify an 868-bp product of the complete ORF0 (Avelar et al. 2019). Nucleic acid extracts from leaf tissue samples of a CLRDV-infected cotton plant and an asymptomatic greenhouse-grown cotton seedling were used as positive and negative controls, respectively. Amplicons of the expected sizes were obtained with the respective primer pairs from all 15 symptomatic plants and the positive control, whereas the five asymptomatic plants tested negative (no amplicon). To verify that the amplicons were virus-specific, two amplicons each per PCR primer pair from two isolates were cloned into the pJET1.2 plasmid vector (Takara Bio USA), and two independent clones per amplicon were determined by Sanger sequencing. After removing the primer sequences, the partial ORF3-5 (268 nucleotides [nt]), partial P0/P1 (694 nt), and complete ORF0 (786 nt) sequences were deposited in GenBank. In pairwise comparisons, the partial ORF3-5-specific sequences from this study (MN506235 to 38) shared 99.6 to 100% nt identity with each other and a maximum nt identity at 99.2 to 99.6% with the corresponding sequences of a CLRDV isolate AL_US from Alabama (MH883236). The P0/P1-specific sequences (MN506239 to 42) shared 94.2 to 99.5% nt identity with each other and shared 94.3 to 99.5% nt identity with corresponding sequences of isolate AL_US from Alabama (MH883237). Similarly, the complete ORF0 sequences derived in this study (MN506243 to 46) shared 93.7 to 100% nt identity with each other and 94.1 to 99.6% nt identity with corresponding sequences of isolate CLRDV isolate Macon_AL (MN071395), also from Alabama. CLRDV is now prevalent in several Asia Pacific and South American countries (Corrêa et al. 2005; Distéfano et al. 2010; Ray et al. 2016) and has also been recently reported from India (Mukherjee et al. 2012). More recently, CLRDV was reported from commercial cotton fields in Alabama, Georgia, and Mississippi (Aboughanem-Sabanadzovic et al. 2019; Avelar et al. 2019; Tabassum et al. 2019). To the best of our knowledge, this is the first report of CLRDV in Texas, where over 45% of U.S. cotton is produced. More recently, additional CLRDV-positive plants were detected from a grower's field in Lubbock County (data not shown), indicating that the virus may be widespread in Texas. Considering the potential threat of CLRDV, studies are needed to determine the biology, epidemiology, and economic impact of the virus under Texas growing conditions.The author(s) declare no conflict of interest.References:Aboughanem-Sabanadzovic, N., et al. 2019. Plant Dis. 103:1798. https://doi.org/10.1094/PDIS-01-19-0017-PDN Link, ISI, Google ScholarAvelar, S., et al. 2019. Plant Dis. 103:592. https://doi.org/10.1094/PDIS-09-18-1550-PDN Link, ISI, Google ScholarCorrêa, R., et al. 2005. Arch. Virol. 150:1357. https://doi.org/10.1007/s00705-004-0475-8 Crossref, ISI, Google ScholarDistéfano, A. J., et al. 2010. Arch. Virol. 155:1849. https://doi.org/10.1007/s00705-010-0764-3 Crossref, ISI, Google ScholarMukherjee, A. K., et al. 2012. New Dis. Rep. 25:22. https://doi.org/10.5197/j.2044-0588.2012.025.022 Crossref, Google ScholarRay, J. D., et al. 2016. Australas. Plant Dis. Notes 11:29. https://doi.org/10.1007/s13314-016-0217-2 Crossref, ISI, Google ScholarSharman, M., et al. 2015. Australas. Plant Dis. Notes 10:24. https://doi.org/10.1007/s13314-015-0174-1 Crossref, ISI, Google ScholarTabassum, A., et al. 2019. Plant Dis. 103:1803. https://doi.org/10.1094/PDIS-12-18-2197-PDN Link, ISI, Google ScholarThe authors are grateful to Sudeep Bag (University of Georgia, Tifton, GA) for providing tissue samples from confirmed CLRDV-positive cotton as a control.The author(s) declare no conflict of interest.DetailsFiguresLiterature CitedRelated Vol. 104, No. 3 March 2020SubscribeISSN:0191-2917e-ISSN:1943-7692 DownloadCaptionPathogenicity of Lasiodiploidia pseudotheobromae in a coffee plant 3 days after inoculation (R. L. Freitas-Lopes et al.). Photo credit: U. P. Lopes. Seedling blight of soybean caused by soilborne pathogens (J. R. Lamichhane et al.). Photo credit: M. I. Chilvers. Metrics Downloaded 3,122 times Article History Issue Date: 3 Mar 2020Published: 5 Jan 2020First Look: 11 Nov 2019Accepted: 6 Nov 2019 Page: 998 Information© 2020 The American Phytopathological SocietyKeywordscottoncotton blue diseaseCotton leafroll dwarf virusPolerovirusaphidsepidemiologyvirus diagnosisThe author(s) declare no conflict of interest.Cited ByCotton Leafroll Dwarf Virus US Genomes Comprise Divergent Subpopulations and Harbor Extensive Variability5 November 2021 | Viruses, Vol. 13, No. 11Effect of Cotton Leafroll Dwarf Virus on Physiological Processes and Yield of Individual Cotton Plants1 October 2021 | Frontiers in Plant Science, Vol. 12Genome analysis of cotton leafroll dwarf virus reveals variability in the silencing suppressor protein, genotypes and genomic recombinants in the USA7 July 2021 | PLOS ONE, Vol. 16, No. 7Natural host range, incidence on overwintering cotton and diversity of cotton leafroll dwarf virus in Georgia USACrop Protection, Vol. 144First Report of Cotton Leafroll Dwarf Virus in Cotton Plants Affected by Cotton Leafroll Dwarf Disease in North CarolinaLindsey D. 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