Artigo Acesso aberto Revisado por pares

Humoral Immunogenicity and Efficacy of a Single Dose of ChAdOx1 MERS Vaccine Candidate in Dromedary Camels

2019; Nature Portfolio; Volume: 9; Issue: 1 Linguagem: Inglês

10.1038/s41598-019-52730-4

ISSN

2045-2322

Autores

Naif Khalaf Alharbi, Ibrahim Qasim, Abdulrahman Almasoud, Haya A. Aljami, Mohamed W. Alenazi, Ali Alhafufi, Omar Aldibasi, Anwar M. Hashem, Samy Kasem, Raed Albrahim, Musaad Aldubaib, Ali Almansour, Nigel Temperton, Alexandra Kupke, Stephan Becker, Ali Abu-obaidah, Ali Alkarar, In‐Kyu Yoon, Esam I. Azhar, Teresa Lambe, Faisal Bayoumi, Ali Aldowerij, Osman H. Ibrahim, Sarah C. Gilbert, Hanan H. Balkhy,

Tópico(s)

Animal Virus Infections Studies

Resumo

Abstract MERS-CoV seronegative and seropositive camels received a single intramuscular dose of ChAdOx1 MERS, a replication-deficient adenoviral vectored vaccine expressing MERS-CoV spike protein, with further groups receiving control vaccinations. Infectious camels with active naturally acquired MERS-CoV infection, were co-housed with the vaccinated camels at a ratio of 1:2 (infected:vaccinated); nasal discharge and virus titres were monitored for 14 days. Overall, the vaccination reduced virus shedding and nasal discharge (p = 0.0059 and p = 0.0274, respectively). Antibody responses in seropositive camels were enhancedby the vaccine; these camels had a higher average age than seronegative. Older seronegative camels responded more strongly to vaccination than younger animals; and neutralising antibodies were detected in nasal swabs. Further work is required to optimise vaccine regimens for younger seronegative camels.

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