Artigo Revisado por pares

Blastocystis sp. Subtype Diversity in Wild Carnivore Species from Spain

2019; Wiley; Volume: 67; Issue: 2 Linguagem: Inglês

10.1111/jeu.12772

ISSN

1550-7408

Autores

Rafael Calero‐Bernal, Mónica Santı́n, Jenny G. Maloney, Manuel Martín‐Pérez, M. Habela, José Luis Fernández-García, Ana M. Figueiredo, Fernando Nájera, Marı́a Jesús Palacios, Marta Mateo, Ana Balseiro, Marta Barral, José F. Lima‐Barberoi, Pamela C. Köster, David Carmena,

Tópico(s)

Coccidia and coccidiosis research

Resumo

Abstract The occurrence and molecular diversity of the stramenopile eukaryote Blastocystis sp. was investigated by PCR and sequencing (Sanger and NGS ) methods in 380 faecal specimens of free‐living carnivores in Spain. Blastocystis sp. was confirmed in 1.6% (6/380) of the specimens analysed. Two samples from a common genet and a fox were successfully subtyped as ST 7 by Sanger. Using NGS , ST 14 was found in a fox and a European polecat, ST 7 in a fox, and two additional foxes presented mixed infections of ST 1/ ST 2/ ST 4 and ST 1/ ST 2/ ST 7, respectively. Wild carnivore species could act as carriers of zoonotic Blastocystis subtypes.

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