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LC-MS determination of catecholamines and related metabolites in red deer urine and hair extracted using magnetic multi-walled carbon nanotube poly(styrene-co-divinylbenzene) composite

2019; Elsevier BV; Volume: 1136; Linguagem: Inglês

10.1016/j.jchromb.2019.121878

1873-376X

Khaled Murtada, Fernando de Andrés, Ismael Galván, Ángel Ríos, Mohammed Zougagh,

Microfluidic and Capillary Electrophoresis Applications

A novel analytical methodology for the extraction and determination of catecholamines (dopamine, epinephrine and norepinephrine) and their metabolites DL-3,4-dihydroxyphenyl glycol and DL-3,4-dihydroxymandelic acid by LC-MS is developed and validated for its application to human and animal urine and hair samples. The method is based on the preliminary extraction of the analytes by a magnetic multi-walled carbon nanotube poly(styrene-co-divinylbenzene) composite. This is followed by a 90%. Accuracy values comprised the range 79.5–109.5% when the analytes were extracted from deer urine samples using the selected MMWCNT-poly(STY-DVB) sorbent. This methodology was applied to real red deer urine and hair samples, and concentrations within range from 0.05 to 0.5 µg mL−1 for norepinephrine and from 1.0 to 44.5 µg mL−1 for its metabolite 3,4-dihydroxyphenyl glycol were calculated. Analyses of red deer hair resulted in high amounts of 3,4-dihydroxyphenyl glycol (0.9–266.9 µg mL−1).