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Cellulase production to obtain biogas from passion fruit (Passiflora edulis) peel waste hydrolysate

2019; Elsevier BV; Volume: 7; Issue: 6 Linguagem: Inglês

10.1016/j.jece.2019.103510

2213-3437

Anderson Felipe Viana da Silva, Liliana Andréa dos Santos, Rebeca Beltrão Valença, Tatiana Souza Porto, Maurı́cio Alves da Motta Sobrinho, Gláucio José Gomes, José Fernando Thomé Jucá, Liliana Andréa dos Santos,

Microbial Metabolic Engineering and Bioproduction

Enzyme usage for valorization of organic and agroindustrial wastes is a biotechnology field that had been increasing due to economic and environmental benefits that add to productive cycles. Enzymatic hydrolysis is one of the possible pre-treatments used with the aim of facilitating lignocellulosic waste biodigestion. This work evaluated cellulase production by Aspergillus japonicus URM5620 and its use as enzymatic pre-treatment on passion fruit peel waste (Passiflora edulis) in order to facilitate the anaerobic biodigestion for biogas generation purposes. Cellulases were produced by submerged fermentation, being maximized using a factorial 2² design, analysing substrate concentration (passion fruit peel) (1.0 to 3.0%) and glucose (0.5 to 1.0%). Enzymatic hydrolyzate obtained from submerged fermentation was biodigested in anaerobic reactor, in a laboratory scale. The highest activities of 1.2 U/mL total cellulase (FPase) and 1.7 U/mL endoglucanase (CMCase) were obtained at 3% of substrate and 1% of glucose. Biogas production from hydrolyzed waste provided methane with a concentration of above 64% by volume after 15 days. Fermentation pre-treatment, using Aspergillus japonicus, was a step that potentially facilitates and accelerates lignocellulosic organic waste biodigestion.