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Metabolic profile of pitaya (Hylocereus polyrhizus (F.A.C. Weber) Britton & Rose) by UPLC-QTOF-MSE and assessment of its toxicity and anxiolytic-like effect in adult zebrafish

2019; Elsevier BV; Volume: 127; Linguagem: Inglês

10.1016/j.foodres.2019.108701

1873-7145

Sandra Machado Lira, Ana Paula Dionísio, Marcelo Oliveira Holanda, Chayane Gomes Marques, Gisele da Silva, Lia Coêlho Correa, Glauber Batista Moreira Santos, Fernando Antônio Pinto de Abreu, Francisco Ernani Alves Magalhães, Emanuela de Lima Rebouças, Jhonyson Arruda Carvalho Guedes, Danielle Ferreira de Oliveira, Maria Izabel Florindo Guedes, Guilherme Julião Zocolo,

Pharmacology and Obesity Treatment

Pitaya is a Cactacea with potential for economic exploitation, due to its high commercial value and its functional components – such betalains, oligosaccharides and phenolic compounds. Although the biological activities of pitaya have been studied using in vivo and in vitro models (anti-inflammatory and antiproliferative activities, as example), its anxiolytic-like effect is still unexplored. Therefore, the aim of this work was to perform a characterization of pulp and peel of pitaya (Hylocereus polyrhizus (F.A.C. Weber) Britton & Rose) using UPLC-QTOF-MSE, and to assess its toxicity and anxiolytic-like effect in adult zebrafish (Danio rerio). The results showed 16 and 15 compounds (in pulp and peel, respectively), including maltotriose, quercetin-3-O-hexoside, and betalains, putatively identified by UPLC-QTOF-MSE. Thus, pitaya pulp and peel showed no toxicity in both models tested (Vero cell lines and zebrafish model, LC50 ˃ 1 mg/mL); and a significant anxiolytic activity, since the treated fish reduced the permanence in the clear zone (Light & Dark Test) compared to that in the control, exhibiting anxiolytic-simile effect of diazepam. However, these effects were reduced by pre-treatment with the flumazenil suggesting that the pulp and peel of pitaya are anxiolytics agents mediated via the GABAergic system. These findings suggested that H. polyrizhus has the potential of developing an alternative plant-derived anxiolytic therapy. In addition, pitaya peel (which is a waste in the food industry) should be regarded as a valuable product, which has the potential as an economic value-added ingredient for anxiety disorders.