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Role of membrane sterol and redox system in the anti-candida activity reported for Mo-CBP2, a protein from Moringa oleifera seeds

2019; Elsevier BV; Volume: 143; Linguagem: Inglês

10.1016/j.ijbiomac.2019.09.142

1879-0003

João Xavier da Silva Neto, Helen Paula Silva da Costa, Ilka M. Vasconcelos, Mirella Leite Pereira, José T.A. Oliveira, Tiago Deiveson Pereira Lopes, Lucas Pinheiro Dias, Nadine Monteiro Salgueiro Araújo, Luiz Francisco Wemmenson Gonçalves Moura, Maurício Fraga van Tilburg, Maria Izabel Florindo Guedes, Larissa Alves Lopes, Eva Gomes Morais, Daniele de Oliveira Bezerra de Sousa,

Piperaceae Chemical and Biological Studies

Plant proteins are emerging as an alternative to conventional treatments against candidiasis. The aim of this study was to better understand the mechanism of action of Mo-CBP2 against Candida spp, evaluating redox system activity, lipid peroxidation, DNA degradation, cytochrome c release, medium acidification, and membrane interaction. Anti-candida activity of Mo-CBP2 decreased in the presence of ergosterol, which was not observed with antioxidant agents. C. albicans treated with Mo-CBP2 also had catalase and peroxidase activities inhibited, while superoxide dismutase was increased. Mo-CBP2 increased the lipid peroxidation, but it did not alter the ergosterol profile in live cells. External medium acidification was strongly inhibited, and cytochrome c release and DNA degradation were detected. Mo-CBP2 interacts with cell membrane constituents, changes redox system enzymes in C. albicans and causes lipid peroxidation by ROS overproduction. DNA degradation and cytochrome c release suggest apoptotic or DNAse activity. Lipid peroxidation and H+-ATPases inhibition may induce the process of apoptosis. Finally, Mo-CBP2 did not have a cytotoxic effect in mammalian Vero cells. This study highlights the biotechnological potential of Mo-CBP2 as a promising molecule with low toxicity and potent activity. Further studies should be performed to better understand its mode of action and toxicity.