First report of grapevine Pinot gris virus in Lebanon and the Middle East
2019; Springer Science+Business Media; Volume: 102; Issue: 2 Linguagem: Inglês
10.1007/s42161-019-00453-w
ISSN2239-7264
AutoresRaied Abou Kubaa, E. Choueiri, Fouad Jreijiri, Yara El Khoury, Pasquale Saldarelli,
Tópico(s)Plant Pathogenic Bacteria Studies
ResumoGrapevine Pinot gris virus .GPGV .Lebanon .RT-PCR Grapevine Pinot gris virus (GPGV) from the genus Trichovirus, family Betaflexiviridae, has been reported from several grape-growing regions of the world in asymptomatic and symptomatic vines showing leaf mottling and deformation (Saldarelli et al. 2017).A total of 108 grapevine samples representing 14 cultivars (Black Magic, Superior Seedless, Midnight Beauty, Black Pearl, Red Globe, Crimson, Vermentino, Marselan, Chardonnay, Petit Verdot, Cabernet Sauvignon, Merlot, Pinot noir, Syrah) were collected randomly from nine vineyards located in Bekaa Valley of Lebanon.Samples were analysed by RT-PCR for the presence of GPGV using two sets of primer pairs (DetF/DetR) targeting the movement protein (MP) and coat protein (CP) genes (Morelli et al. 2014) and primers (GPGVRepF/GPGVRepR) partially covering the RNA-dependent RNA polymerase (RdRp) domain of the GPGV replicase gene (Saldarelli et al. 2015).Results showed that two Black Magic and four Superior Seedless
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