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Polyphenolic Extract (PE) from Olive Oil Exerts a Potent Immunomodulatory Effect and Prevents Graft-versus-Host Disease in a Mouse Model

2019; Elsevier BV; Volume: 26; Issue: 4 Linguagem: Inglês

10.1016/j.bbmt.2019.11.019

1523-6536

Isabel Álvarez‐Laderas, Teresa Lopes Ramos, Mayte Medrano, Rocío Caracuel-García, María Victoria Barbado, Marina Sánchéz‐Hidalgo, Rosario Zamora, Catalina Alarcón‐de‐la‐Lastra, Francisco J. Hidalgo, José I. Piruat, Teresa Caballero‐Velázquez, José Antonio Pérez‐Simón,

T-cell and B-cell Immunology

Polyphenols are a group of chemical substances found in plants, with immunomodulatory, antiproliferative, and anti-inflammatory properties that might be useful in the prophylaxis and treatment of graft-versus-host disease (GVHD). Polyphenolic extract (PE) obtained from extra virgin olive oil (EVOO) decreased the activation and proliferation of activated T cells. In addition, a decreased production of proinflammatory cytokines was observed upon exposure to PE. Western blot assays showed a marked inhibition of Akt phosphorylation and nuclear translocation of NF-κB in activated T cells. In a murine model of acute GVHD, we observed that mice that received a diet supplemented in PE (600 ppm) presented a higher survival rate and lower risk of developing GVHD when compared with the group that received a control diet. Histopathologic examination showed a significantly lower gut involvement in mice receiving PE, with a decrease in proinflammatory cytokines (IL-2, IL-17, and TNF-α) in serum and the reestablishment of butyrate concentration in the gut. In conclusion, PE obtained from EVOO exerted a potent immunomodulatory effect, reducing the activation and proliferation of activated T cells and the production of proinflammatory cytokines. In a murine model of acute GVHD, a PE-supplemented diet reduced the incidence and severity of the disease and increased survival after transplantation.