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Quaternization of angico gum and evaluation of anti-staphylococcal effect and toxicity of their derivatives

2019; Elsevier BV; Volume: 150; Linguagem: Inglês

10.1016/j.ijbiomac.2019.10.126

1879-0003

Ana Karolinne Anastacio de Sousa, Fábio de Oliveira Silva Ribeiro, Taiane Maria de Oliveira, Alyne Rodrigues de Araújo, Jhones do Nascimento Dias, Patrícia Albuquerque, Ildinete Silva-Pereira, Antônia Carla de Jesus Oliveira, Patrick Veras Quelemes, J. R. Leite, Durcilene Alves da Silva,

Seaweed-derived Bioactive Compounds

Modified polysaccharides have been featured as new agents against bacterial infection presenting biocompatibility in their use for medical purposes. In this work, we carried out the quaternization of Angico gum (AG). Quaternized Angico gum derivatives (QAG) were produced using a cationic moiety (3-Chloro-2-hydroxypropyl)trimethylammonium chloride onto the gum backbone. The products were characterized by FT-IR spectroscopy, Zeta potential, elemental analysis, and 1H NMR and degree of substitution (DS) was calculated. QAG were also evaluated for their anti-staphylococcal activity by determining Minimum Inhibitory and Bactericidal concentrations against pathogenic Staphylococcus spp. and by imaging using Atomic Force Microscopy. The hemolysis test and Galleria mellonella model were used to assess toxicity of gums. Our results showed that derivatives who presented highest DS (QAG-A3, 0.48 and QAG-B, 0.54) showed more effective antibacterial activity against tested bacteria, biocompatibility with erythrocytes and non-toxicity in G. mellonella model.