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Biotechnological potential of a novel tannase-acyl hydrolase from Aspergillus sydowii using waste coir residue: Aqueous two-phase system and chromatographic techniques

2019; Elsevier BV; Volume: 23; Linguagem: Inglês

10.1016/j.bcab.2019.101453

1878-8181

Kátia K.S. A. Albuquerque, Wendell Albuquerque, Romero Marcos Pedrosa Brandão Costa, Juanize Matias da Silva Batista, Daniela de Araújo Viana Marques, Raquel Pedrosa Bezerra, Polyanna Nunes Herculano, Tatiana Souza Porto,

Metal-Organic Frameworks: Synthesis and Applications

Aspergillus is a large tannase-producing genus able to provide enzymes which hydrolyse condensed tannins in waste coir residues. In this work, a tannase-acyl hydrolase (TAH) obtained from Aspergillus sydowii (SIS 25) was extracted and purified by PEG/citrate aqueous two-phase system (ATPS), biochemically characterized and applied for detannification of green tea. A statistical design (23) was used to evaluate the influence of the variables: molar mass of PEG (1000–6000 g/mol), PEG concentration (20.0–24.0% w/w) and sodium citrate concentration (15–20% w/w) at pH 6.0. ATPS efficiency was confirmed by obtaining two major chromatographic peaks (by size exclusion chromatography on a Superdex-G75 at 215 nm) with a unique peak responsible for the hydrolysis of 44% of the phenolic compounds in green tea. This study is focused on the obtention of a microbial tannase purified by ATPS, with potential for debittering and clarifying fruit juices.