Produção Nacional
Revisado por pares
CdTe-GSH as luminescent biomarker for labeling the larvicidal action of WSMoL lectin in Aedes aegypti larvae
2019; Elsevier BV; Volume: 187; Linguagem: Inglês
10.1016/j.colsurfb.2019.110672
ISSN1873-4367
AutoresCamila Caroline Lopes Arruda, Denilson V. Freitas, Maria Aparecida Barreto Lopes Seabra, Francisco Humberto Xavier Júnior, Regina Célia Bressan Queiroz de Figueiredo, Thiago Henrique Napoleão, Patrícia Maria Guedes Paiva, Daniela Maria do Amaral Ferraz Navarro, Marcelo Navarro,
Tópico(s)Chalcogenide Semiconductor Thin Films
ResumoMosquito-borne arboviruses compromise human health worldwide. Due to resistance to chemical insecticides, natural compounds have been studied to combat mosquitoes. Previous works have demonstrated a larvicidal activity of the water-soluble Moringa oleifera lectin (WSMoL) against Aedes aegypti, suggesting a mechanism of action based on the interaction between lectin and chitin present in the larvae's peritrophic matrix. In this work, it was investigated the WSMoL activity against Aedes aegypti larvae, by using luminescent bioconjugates of WSMoL conjugated to l-glutathione capped CdTe quantum dots. The conjugation was confirmed by ITC experiments, presenting high enthalpy associated to hydrogen bond interactions between nanoparticles and lectins. The bioconjugate luminescence stability was evaluated by the quantum yield (QY) at different pHs, ionic strengths and heat treatment time. The best parameters reached were pH 7.0, absence of electrolytes and heat treatment, giving QY = 4.4 %. The larvae were exposed to the bioconjugates and analyzed by confocal and fluorescence microscopy. CdTe-WSMol were detected along the entire midgut tract, suggesting a strong interaction with peritrophic matrix and lumen of the Aedes aegypti.
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