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Overexpression, purification, enzymatic and microscopic characterization of recombinant mycobacterial F-ATP synthase

2019; Elsevier BV; Volume: 522; Issue: 2 Linguagem: Inglês

10.1016/j.bbrc.2019.11.098

1090-2104

Wuan Geok Saw, Chui Fann Wong, Thomas Dick, Gerhard Grüber,

RNA and protein synthesis mechanisms

The F-ATP synthase is an essential enzyme in mycobacteria, including the pathogenic Mycobacterium tuberculosis. Several new compounds in the TB-drug pipeline target the F-ATP synthase. In light of the importance and pharmacological attractiveness of this novel antibiotic target, tools have to be developed to generate a recombinant mycobacterial F1FO ATP synthase to achieve atomic insight and mutants for mechanistic and regulatory understanding as well as structure-based drug design. Here, we report the first genetically engineered, purified and enzymatically active recombinant M. smegmatis F1FO ATP synthase. The projected 2D- and 3D structures of the recombinant enzyme derived from negatively stained electron micrographs are presented. Furthermore, the first 2D projections from cryo-electron images are revealed, paving the way for an atomic resolution structure determination.