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Polyphenol Characterization in Red Beverages of Carapa procera (D.C.) Leaf Extracts

2019; Multidisciplinary Digital Publishing Institute; Volume: 5; Issue: 4 Linguagem: Inglês

10.3390/beverages5040068

2306-5710

Félix Adjé, N Emmanuel, Kisselmina Youssouf Kone, Emmanuelle Meudec, Augustin Amissa Adima, Paul R. Lozano, Yves Lozano, Émile M. Gaydou,

Essential Oils and Antimicrobial Activity

The red aqueous beverages of Carapa procera (D.C.) leaf extracts were investigated for their polyphenol contents using HPLC-DAD, HPLC-ESI-MS, and semipreparative HPLC. Polyphenols were extracted, clarified, and concentrated using a multistep process including ultrasound-assisted extraction (UAE), cross-flow microfiltration (CFM), and reverse osmosis (RO). On the basis of analytical and semipreparative chromatographic techniques, 12 phenolic compounds were identified and quantified for the first time: 2 anthocyanins (cyanidin 3-O-glucoside and cyanidin 3-O-rutinoside), 5 phenolic acids (protocatechuic, the three caffeoylquinic isomers, and coumaroylquinic acid), and 5 flavonols (quercetin 3-O-rutinoside, quercetin 3-O-galactoside, quercetin 3-O-glucoside, quercetin aglycone, and kaempferol 3-O-rutinoside). From the concentrated extract, it was possible to recover for anthocyanins (28.4 ± 0.3 µmol L−1 cyanidin equivalents) the two glycosides of cyanidin, for flavonols (1587 ± 3 µmol L−1 quercetin equivalents) the two glycosides of quercetin and kaempferol, and for phenolic acids (3650 ± 10 µmol L−1 gallic acid equivalents) chlorogenic and protocatechuic acids.