Produção Nacional
Revisado por pares
Antifungal activity of different molecular weight chitosans against planktonic cells and biofilm of Sporothrix brasiliensis
2019; Elsevier BV; Volume: 143; Linguagem: Inglês
10.1016/j.ijbiomac.2019.12.031
ISSN1879-0003
AutoresLana Glerieide Silva Garcia, Gláucia Morgana de Melo Guedes, Xhaulla Maria Quariguasi Cunha Fonseca, Waldemiro Aquino Pereira-Neto, Débora de Souza Collares Maia Castelo‐Branco, José Júlio Costa Sidrim, Rossana de Aguiar Cordeiro, Marcos Fábio Gadelha Rocha, Rodrigo Silveira Vieira, Raimunda Sâmia Nogueira Brilhante,
Tópico(s)Antimicrobial agents and applications
ResumoSporotrichosis, caused by Sporothrix schenckii complex species, is the most prevalent subcutaneous mycosis in many areas of Latin America. Chitosan has been used as an antifungal agent; however the effects of the molecular weight (MW) of chitosan (i.e. high (HMW), medium (MMW) and low (LMW) molecular weight chitosan) on S. brasiliensis has not been well described, particularly on biofilms. Effects on the planktonic form activity of S. brasiliensis were quantified by broth microdilution, while anti-biofilm activity was quantified by measuring metabolic activity via XTT (2,3-bis(2-methoxy-4-nitro-5-sulfophenyl)-5-[(phenylamino)carbonyl]-2H-tetrazolium hydroxide and biomass formation (crystal violet). The molecular weight of chitosan modulated its effect on the planktonic form of S. brasiliensis, presenting lower MIC values for LMW chitosan. With regards both the adhesive and mature phases of biofilm, the LMW chitosan reduced biomass and metabolic activity most effectively. This study confirms the effects of the molecular weight and deacetylation degree of chitosan on its antifungal properties for potentially pathogenic fungi.
Referência(s)