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Fast and accurate long-read assembly with wtdbg2

2019; Nature Portfolio; Volume: 17; Issue: 2 Linguagem: Inglês

10.1038/s41592-019-0669-3

1548-7105

Jue Ruan, Heng Li,

Bacteriophages and microbial interactions

Existing long-read assemblers require thousands of central processing unit hours to assemble a human genome and are being outpaced by sequencing technologies in terms of both throughput and cost. We developed a long-read assembler wtdbg2 (https://github.com/ruanjue/wtdbg2) that is 2–17 times as fast as published tools while achieving comparable contiguity and accuracy. It paves the way for population-scale long-read assembly in future. Wtdbg2 assembles genomes with comparable contiguity and accuracy to existing tools using long-read sequencing data, and is several times faster, especially for large genomes.