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Molecular study on Cytolysins, Adhesins, Siderophores and Efflux pump among Cervico-Vaginal Escherichia coli

2019; Foundation for Enviromental Protection and Research; Volume: 13; Issue: 2 Linguagem: Inglês

1307-9867

Marwa M. Al-Khaqani, Hussein O.M. Al-Dahmoshi, Mourouge Saadi Alwash,

Urinary Tract Infections Management

Background: Bacterial vaginosis is an important female reproductive tract infection. It push a threat of miscarriage or fertility-related problems if it untreated. Cervico-vaginal E. coli (CVEC) is the common cause agent of microbial vaginosis. It equipped by a set of adhesins, immune-disguise protein, cytolysins, siderophores and metal efflux pump that enable them from establishing and endure the infection. Methodology: One hundred fifty seven (157) vaginal swabs were collected from female clinically diagnosed with bacterial vaginitis. The swab was put in tubes of broth of Brain heart infusion (BHIB) to keep them vital and non-dry until being taken to the laboratory and the inoculated on MacConkey agar Eosin methylene blue agar. The only pinky colony with green-metallic shine, on MacConkey agar Eosin methylene blue agar respectively, were selected for study. Polymerase chain reaction using specific primer pairs was used to investigate the virulence factors at molecular level. Results: The results revealed that, out of (157) clinical samples only 32 (20.38%) isolates were belonged to E. coli. Regarding the adhesins the results revealed that FimH adhesin was the most common virulence factor and was discovered in 25( 78.13%) of CVEC strains, followed by FocG 23(71.87% ), PapGII 22(68.75%), GafD 12(37.5%) and SfaS 8(21.87%) while PapGI and PapGIII not detected in the current study. Concern the cytolysins the results showed that 25(78.13%), 27(84.38%) of CVEC isolates possess hemolysin gene (hlyA) and phospholipase A gene (pldA). The investigation of both kpsMT-K1 and kpsMT-K5 genes. The results revealed that kpsMT-K1 were predominant and compile 10(31.25%), while kpsMT-K5 (which encodes for K5 capsule) were not detected. Aerobactin gene (iucD) were detected in 24(75%) of CVEC isolates. The presence of cusC gene (as a representative for cusCFBA operon responsible for copper/silver efflux) revealed that all CVEC have cusC 32(100%). Conclusion: The current study conclude possession of CVEC for a sets of virulence effectors that enable them from establish the infection, dissemination, immune system evading and sustainability of infection.