Produção Nacional
Revisado por pares
Histopathological, genotoxic, and behavioral damages induced by manganese (II) in adult zebrafish
2019; Elsevier BV; Volume: 244; Linguagem: Inglês
10.1016/j.chemosphere.2019.125550
ISSN1879-1298
AutoresGabriela Zimmermann Prado Rodrigues, Luciane Beatris Mentges Staudt, Marina Griebeler Moreira, Thainá Garbino dos Santos, Mateus Santos de Souza, Caio Júnior Lúcio, Jenifer Panizzon, Juliana Machado Kayser, Leonardo Airton Ressel Simões, Ana Luíza Ziulkoski, Carla Denise Bonan, Diogo Lösch de Oliveira, Günther Gehlen,
Tópico(s)Zebrafish Biomedical Research Applications
ResumoManganese is a metal often found as an environmental pollutant and very associated with neurological disorders when in high concentrations. However, little is known about the effects that this contaminant can cause when in environmentally relevant concentrations and occurrence, that is, much lower than those commonly studied. So, the aim of the study was to evaluate the effects that environmentally relevant concentrations of this metal would cause in different zebrafish organs (brain, liver, and blood). Acute 96-h and chronic 30-day exposures were performed using the manganese chloride salt as a pollutant. Behavioral alterations of anxiogenic type were observed in the animals after chronic exposures to 4.0 mg L−1 MnCl2, which traveled a greater distance at the bottom of the aquarium. This may be associated with neuronal damages in the telencephalic region responsible for motor and cognitive activity of the fish, observed in animals from the same exposure. In addition, hepatic histopathological damage as vacuolization of hepatocytes and genotoxic damage, identified by comet assay and micronucleus test, was also observed after acute and chronic exposure, especially at the highest pollutant concentrations (8.0 and 16.0 mg L−1 in acute exposure, and 4.0 mg L−1 in chronic exposure. The study reinforces the risk that environmental pollutants pose to the ecosystem, even in low concentrations.
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