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Genetic diversity of Acer pseudoplatanus L. populations from Transylvania

2019; Springer Science+Business Media; Volume: 42; Issue: 4 Linguagem: Inglês

10.1007/s40415-019-00567-6

1806-9959

Florin Alexandru REBREAN, Adrian Füstös, Ioan Tǎut, Katalin Szabo, Monica Hârța, Doru Pamfil, Mihaela Simona Rebrean, Tudor Sălăgean,

Botanical Studies and Applications

Acer pseudoplatanus L. is a species with high adaptability, widespread across the Carpathian Mountains, at altitudes from 700 to 1600 m. Due to this fact, this may be encountered into stands situated in diverse climatic conditions. In order to determine the genetic variability of forest genetic resources for in situ conservation purposes, five populations of A. pseudoplatanus with different geographic provenances were examined through sequence-related amplified polymorphism molecular marker technique. A total of 75 individuals originated from natural stands with an important role for forest seeds reserves were evaluated in terms of genetic diversity indices. Out of 20 primer combinations tested, eight presented high grades of polymorphism and clearly visible bands. Shannon's information index was situated between 0.331 (Bistriţa) and 0.435 (Cluj) with an average of 0.3963. The mean value of Nei's gene diversity was 0.259, and a low level of genetic differentiations between populations was calculated (Gst 0.1139). Cluster analysis of the genetic distances indicates two groups with a genetic distance of 0.65. Three-dimensional plot with eigenvectors of principal coordinates analysis (PCoA) of the five provenances was constructed, and the PCoA results confirmed the number of UPGMA clusters. The result of Mantel test indicates no significant correlation between the geographic distances and the genetic distances.