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Essential oil from Eugenia stipitata McVaugh leaves has antinociceptive, anti-inflammatory and antipyretic activities without showing toxicity in mice

2019; Elsevier BV; Volume: 144; Linguagem: Inglês

10.1016/j.indcrop.2019.112059

1872-633X

Wêndeo Kennedy Costa, João Ricardhis Saturnino de Oliveira, Alisson Macário de Oliveira, Izabelly Bianca da Silva Santos, Rebeca Xavier da Cunha, Anderson Felipe Soares de Freitas, Janderson Weydson Lopes Menezes da Silva, Valquíria Bruna Guimarães Silva, Júlio César Ribeiro de Oliveira Farias de Aguiar, Alexandre Gomes da Silva, Daniela Maria do Amaral Ferraz Navarro, Vera Lúcia de Menezes Lima, Márcia Vanusa da Silva,

Phytochemistry and Bioactive Compounds

Eugenia stipitata is a Brazilian plant species used in traditional medicine for a wide range of ailments. Considering the biotechnological potential of herbal oils, in this study an essential oil was evaluated for toxicity, antinociceptive, antipyretic, and anti-inflammatory activity in mice model. The essential oil from Eugenia stipitata (EsEO) leaves was obtained by hydrodistillation and analyzed by gas chromatography-mass spectrometry. Abdominal contortion tests were performed to evaluate its antinociceptive activity. For estimating its anti-inflammatory activity, tests of paw edema, peritonitis, and protein denaturation were performed. The antipyretic effect was assessed with a yeast-induced pyrexia model. Artemia salina was used for environmental biomonitoring and preliminary toxicity. To analyse the safety of use; acute toxicity and hemolytic activity were performed. Majority of the compounds were identified by guaiol (13.77%), trans-caryophyllene (11.36%), and β-eudesmol (8.13%) e γ-eudesmol (6.55%). Essential Oil from E. stipitata promoted mortality of A. salina nauplii, with an LC50 of 812.45 μg/mL. No mice death(s) was noted with oral administration of the essential oil at 2000 and 5000 mg/kg, respectively; additionally, no alterations were observed with biochemical, hematological, and histopatological analyses. Markedly reduced abdominal contortion (54.1–56.6% inhibition) at all doses as compared with the negative control. Reduction in inflammation was also seen. In the paw edema test, a significant decrease in edema (88.66–96.94%) was observed. Whereas, in peritonitis; a reduction in the migration of total leukocytes (76.8–86.5%) and neutrophils (74.5–79.6%) was noted. Moreover, EsEO was capable of inhibiting protein denaturation (74.39–82.83%) as compared with the control (45.02–100%) at the concentrations evaluated. It also exerted an antipyretic effect after the first 1 h of evaluation, until the end of observation duration (4 h). The results suggested EsEO as a promising source of natural antinociceptive, anti-inflammatory, and antipyretic constituent for application in food and pharmaceutical industries.