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MicroRNA Expression in Cutaneous Lupus: A New Window to Understand Its Pathogenesis

2019; Hindawi Publishing Corporation; Volume: 2019; Linguagem: Inglês

10.1155/2019/5049245

1466-1861

Silvia Méndez‐Flores, Janette Furuzawa‐Carballeda, Gabriela Hernández‐Molina, Gustavo Ramírez-Martínez, Nora E. Regino-Zamarripa, Blanca Ortíz-Quintero, Luis Jiménez-Álvarez, Alfredo Cruz‐Lagunas, Joaquı́n Zúñiga,

Circular RNAs in diseases

The role of miRNAs in the pathogenesis of cutaneous lupus has not been studied.It was to assess the levels of a selected panel of circulating miRNAs that could be involved in the regulation of the immune response, inflammation, and fibrosis in cutaneous lupus.It was a cross-sectional study. We included 22 patients with subacute (SCLE) and 20 with discoid (DLE) lesions, and 19 healthy donors (HD). qRT-PCR for miRNA analysis, flow cytometry in peripheral blood, and skin immunohistochemistry were performed to determine the distribution of CD4 T cells and regulatory cells and their correlation with circulating miRNAs.miR-150, miR-1246, miR-21, miR-23b, and miR-146 levels were downregulated in SCLE vs. HD. miR-150, miR-1246, and miR-21 levels were downregulated in DLE vs. HD. miR-150, miR-1246, and miR-21 levels were downregulated in DLE γ+ with miR-1246 in SCLE, whereas CD123+/CD196+/IDO+ cells were positively associated with miR-150 in DLE. In the tissue, CD4+/IL-4+ and CD20+/IL-10+ cells were positively associated with miR-21 and CD4+/IFN-γ+ with miR-1246 in SCLE, whereas CD123+/CD196+/IDO+ cells were positively associated with miR-150 in DLE. In the tissue, CD4+/IL-4+ and CD20+/IL-10+ cells were positively associated with miR-21 and CD4+/IFN-β, thyroid hormone, and cancer signaling pathways were shared between miR-21, miR-31, miR-23b, miR-146a, miR-1246, and miR-150.A downregulation of miR-150, miR-1246, and miR-21 in both CLE varieties vs. HD. miR-150, miR-1246, and miR-21 levels were downregulated in DLE.