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Dual-Peptide-Functionalized Nanofibrous Scaffolds Recruit Host Endothelial Progenitor Cells for Vasculogenesis to Repair Calvarial Defects

2019; American Chemical Society; Volume: 12; Issue: 3 Linguagem: Inglês

10.1021/acsami.9b21434

1944-8252

Li Li, Wanqian Liu, Yulan Zhao, Pingping Ma, Shenfang Zha, Peixin Chen, Hongwei Lu, Xiaorui Jiang, Shuang Wan, Jiangming Luo, Qijie Dai, Junxian Hu, Yohanes Kristo Sugiarto Utomo, Xinyun Han, Zhengwei Yang, Li Yang, Qingyi He,

Protease and Inhibitor Mechanisms

Vasculogenesis (de novo formation of vessels) induced by endothelial progenitor cells (EPCs) is requisite for vascularized bone regeneration. However, there exist few available options for promoting vasculogenesis within artificial bone grafts except for exogenous EPC transplantation, which suffers from the source of EPC, safety, cost, and time concerns in clinical applications. This study aimed at endogenous EPC recruitment for vascularized bone regeneration by using a bioinspired EPC-induced graft. The EPC-induced graft was created by immobilizing two bioactive peptides, WKYMVm and YIGSR, on the surface of poly(ε-caprolactone) (PCL)/poliglecaprone (PGC) nanofibrous scaffolds via a polyglycolic acid (PGA)-binding peptide sequence. Remarkable immobilization efficacy of WKYMVm and YIGSR peptides and their sustained release (over 14 days) from scaffolds were observed. In vivo and in vitro studies showed robust recruitment of EPCs, which subsequently contributed to early vasculogenesis and ultimate bone regeneration. The dual-peptide-functionalized nanofibrous scaffolds proposed in this study provide a promising therapeutic strategy for vasculogenesis in bone defect repair.