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Synergistic Anticandidal Activity of Etomidate and Azoles Against Clinical Fluconazole-Resistant Candida Isolates

2019; Future Medicine; Volume: 14; Issue: 17 Linguagem: Inglês

10.2217/fmb-2019-0075

1746-0921

Lívia Gurgel do Amaral Valente Sá, Cecília Rocha da Silva, S Campos de Rosana, João B A de Neto, Sandro Serpa, Francisca Bruna Stefany Aires do Nascimento, Fátima DD Barroso, Lisandra J da Silva, Helaine Almeida Queiroz, Thiago M Cândido, Daniel Sampaio Rodrigues, Amanda C Leitão, Manoel Odorico de Moraes, Bruno C. Cavalcanti, Hélio Vitoriano Nobre Júnior,

Pneumocystis jirovecii pneumonia detection and treatment

Aim: The purpose of this study was to evaluate the effect of etomidate alone and in combination with azoles on resistant strains of Candida spp. in both planktonic cells and biofilms. Materials & methods: The antifungal activity of etomidate was assessed by the broth microdilution test; flow cytometric procedures to measure fungal viability, mitochondrial transmembrane potential, free radical generation and cell death; as well detection of DNA damage using the comet assay. The interaction between etomidate and antifungal drugs (itraconazole and fluconazole) was evaluated by the checkerboard assay. Results: Etomidate showed antifungal activity against resistant strains of Candida spp. in planktonic cells and biofilms. Etomidate also presented synergism with fluconazole and itraconazole in planktonic cells and biofilms. Conclusion: Etomidate showed antifungal activity against Candida spp., indicating that it is a possible therapeutic alternative.