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Characterization of ESBL-Producing Enterobacteria from Fruit Bats in an Unprotected Area of Makokou, Gabon

2020; Multidisciplinary Digital Publishing Institute; Volume: 8; Issue: 1 Linguagem: Inglês

10.3390/microorganisms8010138

2076-2607

Pierre Philippe Mbehang Nguema, Richard Onanga, Guy Roger Ndong Atome, Jean Constant Obague Mbeang, Arsène Mabika Mabika, Moussa Yaro, Manon Lounnas, Yann Dumont, Zaidi Fatma Zohra, Sylvain Godreuil, François Bretagnolle,

Gut microbiota and health

In Gabon, terrestrial mammals of protected areas have been identified as a possible source of antibiotic-resistant bacteria. Some studies on antibiotic resistance in bats have already been carried out. The main goal of our study was to detect extended-spectrum beta-lactamases (ESBLs) that are produced by enterobacteria from bats in the Makokou region in Gabon. Sixty-eight fecal samples were obtained from 68 bats caught in the forests located 1 km from the little town of Makokou. After culture and isolation, 66 Gram-negative bacterial colonies were obtained. The double-disk diffusion test confirmed the presence of ESBLs in six (20.69%) Escherichia coli isolates, four (13.79%) Klebsiella pneumoniae isolates, and one (3.45%) Enterobacter cloacae isolate. The analysis based on the nucleotide sequences of the ESBL resistance genes showed that all cefotaximase-Munichs (CTX-Ms) were CTX-M-15 and that all sulfhydryl variables (SHVs) were SHV-11: 54.54% ESBL (CTX-M-15)-producing E. coli, 9.09% ESBL (CTX-M-15)-producing K. pneumoniae, 27.27% ESBL (CTX-M-15, SHV-11)-producing K. pneumoniae, and 9.09% ESBL (CTX-M-15)-producing E. cloacae. This study shows for the first time the presence of multiresistant ESBL-producing enterobacteria in fruit bats in Makokou.