Produção Nacional
Revisado por pares
First Report of Geotrichum candidum Causing Sour-Rot of Melon in Brazil
2020; American Phytopathological Society; Volume: 104; Issue: 5 Linguagem: Inglês
10.1094/pdis-11-19-2484-pdn
ISSN1943-7692
AutoresB. de A. Halfeld-Vieira, D. Terao, K. de L. Nechet,
Tópico(s)Yeasts and Rust Fungi Studies
ResumoHomePlant DiseaseVol. 104, No. 5First Report of Geotrichum candidum Causing Sour-Rot of Melon in Brazil PreviousNext DISEASE NOTES OPENOpen Access licenseFirst Report of Geotrichum candidum Causing Sour-Rot of Melon in BrazilB. A. Halfeld-Vieira, D. Terao, and K. L. NechetB. A. Halfeld-Vieira†Corresponding author: B. A. Halfeld-Vieira; E-mail Address: bernardo.halfeld@embrapa.brhttp://orcid.org/0000-0003-4115-0155Embrapa Meio Ambiente, Jaguariúna, BrazilSearch for more papers by this author, D. TeraoEmbrapa Meio Ambiente, Jaguariúna, BrazilSearch for more papers by this author, and K. L. Nechethttp://orcid.org/0000-0002-6746-0576Embrapa Meio Ambiente, Jaguariúna, BrazilSearch for more papers by this authorAffiliationsAuthors and Affiliations B. A. Halfeld-Vieira † D. Terao K. L. Nechet Embrapa Meio Ambiente, Jaguariúna, Brazil Published Online:17 Mar 2020https://doi.org/10.1094/PDIS-11-19-2484-PDNAboutSectionsSupplemental ToolsAdd to favoritesDownload CitationsTrack Citations ShareShare onFacebookTwitterLinked InRedditEmailWechat Melon (Cucumis melo) is the most exported fruit in Brazil (224,000 tonnes), and the production increased by 10% in the past growing season. Currently, 22,000 ha are devoted to its cultivation in the country, and production has reached above 500,000 tonnes. The production is concentrated on the northeast semiarid area, particularly in Chapada do Apodi, between Rio Grande do Norte and Ceará States. Rio Grande do Norte State is responsible for 70% of Brazilian production of melon (Brazilian Fruit Yearbook 2018). In 2017, an incidence of a little over 50% of rotten fruits in a highly representative commercial field was detected in Canary melons from Mossoró municipality, Rio Grande do Norte. Sour-rot symptoms were observed mainly on the top of the fruit, at the floral insertion site. The lesions were dark and eventually progressed to epidermal cracks. The associated fungus was consistently isolated from symptomatic fruits and grown on potato dextrose agar (PDA), presenting white, flat smooth colonies with pale yellow reverse side. Mycelia were hyaline, with dichotomously branched septate hyphae. Arthroconidia were in long chains, hyaline, 1-celled, cylindrical to subglobose (11.4 to 4.5 × 6.6 to 4.3 µm). Morphological features were consistent with Geotrichum candidum description (de Hoog et al. 1986). The genomic DNA was extracted with UltraClean Microbial DNA Isolation Kit (Mobio, Carlsbad, CA) and the internal transcribed spacer (ITS) was amplified using the pair of primers ITS1 and ITS4 (Gardes and Bruns 1993). The obtained ITS sequence (GenBank accession no. MH185953) showed 100% identity with G. candidum (KF112070.1 and LC054542.1), corroborating the morphological identification. The isolate was deposited at the Collection of Microorganisms of Agricultural and Environmental Importance at Embrapa Meio Ambiente in Jaguariúna, São Paulo, Brazil (CMAA) as G. candidum CMAA-1616. To fulfill Koch’s postulates, Canary melon fruit were disinfected with 1% sodium hypochlorite solution and rinsed in sterile distilled water. Thereafter, 5 µl of pure conidia suspension of Geotrichum candidum (5 × 107 conidia ml−1) was deposited on a 3-mm diameter, 2-mm depth wound previously made on the melon fruit peel using a sterile nail. Eight fruits with four wounds per fruit were used. After inoculation, fruits were placed in a humid chamber at 23 ± 2°C for 12 h, and thereafter maintained at room temperature (22 ± 2°C and 70 ± 2% relative humidity). The development of symptoms was observed for 5 days. The causal agent was reisolated from the rotten peel and the pure culture of G. candidum was obtained. This is the first report of G. candidum on melon in Brazil. Sour-rot is a well-known disease of melon in other countries (Ceponis 1966) and can be listed as a potential postharvest disease for melon fruit in Brazil.The author(s) declare no conflict of interest.References:Brazilian Fruit Yearbook. 2018. Page 79. Gazeta Santa Cruz, Santa Cruz. http://www.editoragazeta.com.br/sitewp/wp-content/uploads/2018/04/FRUTICULTURA_2018_dupla.pdf Google ScholarCeponis, M. J. 1966. Plant Dis. Rep. 50:222. Google Scholarde Hoog, G. S., et al. 1986. Stud. Mycol. 29:1. Google ScholarGardes, M., and Bruns, T. D. 1993. Mol. Ecol. 2:113. https://doi.org/10.1111/j.1365-294X.1993.tb00005.x Crossref, ISI, Google ScholarThe author(s) declare no conflict of interest.Funding: The authors thank the São Paulo Research Foundation (Fundação de Amparo à Pesquisa do Estado de São Paulo - FAPESP: 2018/25318-7) for financial support. Bernardo de Almeida Halfeld-Vieira thanks the National Council for Technological and Scientific Development (CNPq) for his research productivity fellowship (Proc. 303396/2018-0).DetailsFiguresLiterature CitedRelated Vol. 104, No. 5 May 2020SubscribeISSN:0191-2917e-ISSN:1943-7692 DownloadCaptionSymptoms observed in the field on zucchini plants caused by Fusarium solani f. sp. cucurbitae (A. Pérez-Hernández et al.). Photo credit: J. M. Gómez-Vázquez. Peach tree with excavated root collar (S. B. Miller et al.). Photo credit: G. Schnabel. Metrics Article History Issue Date: 3 May 2020Published: 17 Mar 2020First Look: 22 Jan 2020Accepted: 21 Jan 2020 Pages: 1537-1537 Information© 2020 The American Phytopathological SocietyKeywordsGeotrichum candidumCucumis melosour-rotBrazilThe author(s) declare no conflict of interest.Cited byPotential of bioaugmentation of heavy metal contaminated soils in the Zambian Copperbelt using autochthonous filamentous fungi1 December 2022 | Frontiers in Microbiology, Vol. 13Geotrichum candidumCABI Compendium, Vol. CABI CompendiumFirst Report of Geotrichum candidum Causing Sour Rot of Peach in ChinaR. X. Lu, Z. Wang, Y. J. Zhai, R. Y. Hong, F. Shen, W. X. Jin, C. L. Zheng, and H. Q. Ma22 October 2021 | Plant Disease, Vol. 105, No. 10First Report of Geotrichum candidum Causing Postharvest Sour Rot on Kiwifruits in ChinaHao Cheng, Wei Tang, Hanyang Wang, Qianwen Liu, Huanhuan Li, and Yongsheng Liu7 April 2021 | Plant Disease, Vol. 105, No. 5
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