Mechanism of Interference by Hemolysis in Immunoassays and Requirements for Sample Quality
1998; American Association for Clinical Chemistry; Volume: 44; Issue: 12 Linguagem: Inglês
10.1093/clinchem/44.12.2554
ISSN1530-8561
Autores Tópico(s)Thermal Regulation in Medicine
ResumoCardiac troponin I (cTnI) is considered organ-specific, and few circumstances other than heart muscle injury produce false increases in plasma. We encountered unexpectedly high cTnI in a two-site, enzyme-linked immunoassay using polyclonal goat antibody (1) when by mistake, two visually clear but grossly hemolyzed samples from one patient were not rejected by the laboratory. Hemoglobin (Hb) reportedly is not an interferent in the test: “… levels of the following do not appear to interfere… hemoglobin 2,000 mg/dL” (2). Elsewhere, the manufacturer states that: “Plasma samples should be clear to straw in color” (3) and “… strongly hemolyzed… samples should be avoided” (4). No data are …
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