First Report of Phytophthora sansomeana Causing Root Rot of Soybean in South Dakota.
2020; American Phytopathological Society; Volume: 104; Issue: 6 Linguagem: Inglês
10.1094/pdis-09-19-2005-pdn
ISSN1943-7692
AutoresC. Tande, Anne E. Dorrance, D. Schwarzrock, E. Mahecha, Emmanuel Byamukama,
Tópico(s)Plant Disease Resistance and Genetics
ResumoHomePlant DiseaseVol. 104, No. 6First Report of Phytophthora sansomeana Causing Root Rot of Soybean in South Dakota. PreviousNext DISEASE NOTES OPENOpen Access licenseFirst Report of Phytophthora sansomeana Causing Root Rot of Soybean in South Dakota.C. Tande, A. E. Dorrance, D. Schwarzrock, E. Mahecha, and E. ByamukamaC. TandeAgronomy, Horticulture, and Plant Science Department, South Dakota State University, Brookings, SD 57007, A. E. Dorrancehttp://orcid.org/0000-0003-4138-6707Dept. of Plant Pathology, The Ohio State University, Wooster, OH 44691, D. SchwarzrockAgronomy, Horticulture, and Plant Science Department, South Dakota State University, Brookings, SD 57007, E. MahechaAgronomy, Horticulture, and Plant Science Department, South Dakota State University, Brookings, SD 57007, and E. Byamukama†Corresponding author: E. Byamukama; E-mail Address: ebyamukama@gmail.comhttp://orcid.org/0000-0003-4914-5457Agronomy, Horticulture, and Plant Science Department, South Dakota State University, Brookings, SD 57007AffiliationsAuthors and Affiliations C. Tande1 A. E. Dorrance2 D. Schwarzrock1 E. Mahecha1 E. Byamukama1 † 1Agronomy, Horticulture, and Plant Science Department, South Dakota State University, Brookings, SD 57007 2Dept. of Plant Pathology, The Ohio State University, Wooster, OH 44691 Published Online:25 Mar 2020https://doi.org/10.1094/PDIS-09-19-2005-PDNAboutSections ToolsAdd to favoritesDownload CitationsTrack Citations ShareShare onFacebookTwitterLinked InRedditEmailWechat Phytophthora root and stem rot, caused by Phytophthora spp., is an important disease of soybean in South Dakota. During 2016 and 2017, a survey was carried out in South Dakota to determine pathotypes of Phytophthora spp. in order to deploy corresponding host resistance genes. Soil and symptomatic soybean plant samples were collected from 57 soybean fields with a history of Phytophthora root and stem rot in 19 counties of South Dakota. One hundred and forty-one isolates of Phytophthora spp. were baited from soil or isolated from plants following standard protocols (Dorrance et al. 2007). The isolates were transferred to full strength potato dextrose agar (PDA) to check for growth as P. sojae does not grow on this medium (Dorrance et al. 2007). Ten isolates grew on PDA and hence were suspected to be Phythophthora sansomeana, a known Phytophthora spp. on soybean reported in other states. These isolates were transferred to lima bean agar and the colonies on this media were rosaceous and had oogonia (3 to 5 per plate) that measured 35 to 50 µm in diameter after 7 to 10 days (Tang et al. 2010). DNA was extracted from 3-day-old mycelia grown on quarter strength V8 broth for each of the 10 isolates using Wizard Genomic DNA extraction kit (Promega). To confirm the identification of P. sansomeana and rule out P. sojae, a qPCR method described by Rojas et al. (2017) using primers PhyG_ATP9_2F_Tail, PhyG-R6_Tail, Phytophthora genus specific TaqMan probe, P. sojae species specific TaqMan probe, and P. sansomeana species specific TaqMan probe was used. The 10 isolates originally suspected to be positive for P. sansomeana were confirmed by qPCR. A negative check was done using DNA extracted from P. sojae and no amplification was obtained from this sample. Two isolates were arbitrarily selected from the 10 isolates and their DNA was sent for sequencing by Sanger method (Functional Biosciences Inc, Madison WI) after running PCR using the internal transcribed spacer (ITS) region primers ITS6/ITS4. Blasting the sequences of the two isolates through a BLASTn search revealed 100% identities and 100% coverage with ITS sequences of several P. sansomeana isolates including the ex-type isolate accession number MG865585.1. The sequences were deposited in GenBank under reference accession numbers MN497565 and MN497566. Pathogenicity of P. sansomeana was performed in a greenhouse assay. Wheat seed (25 g) was autoclaved twice in 0.95-liter glass jars and 10 to 12 plugs of P. sansomeana fully colonized LBA were added and incubated at room temperature for 10 days. Ten seeds of soybean cultivars Sloan and Codington were sown into 0.47-liter Styrofoam cups containing 5 g of P. sansomeana-infested wheat seed mixed with vermiculite. The same cultivars were sown in cups without inoculum as controls. This was repeated for a total of three times. Ten to 14 days after seeding, soybean plants sown into the cups with inoculum developed brown discoloration on roots. No symptoms developed on plants sown in the control cups. P. sansomeana was isolated from all symptomatic roots using the same procedure above, thus completing Koch’s postulates. The isolate recovered from the roots in the greenhouse was confirmed using qPCR as described above. To our knowledge, this is the first report of P. sansomeana in South Dakota. Further research is needed to determine the extent of the yield loss that P. sansomeana may be causing to soybean and the other crops, such as corn, that may be hosts.The author(s) declare no conflict of interest.References:Dorrance, A. E., et al. 2007. The Plant Health Instructor. doi:10.1094/PHI-I-2007-0830-07 Google ScholarRojas, et al. 2017. Plant Dis. 101:1171. Link, ISI, Google ScholarTang, Q. H., et al. 2010. Plant Dis. 94:378. https://doi.org/10.1094/PDIS-94-3-0378A Link, ISI, Google ScholarThe author(s) declare no conflict of interest.Funding: Funding for this work was provided by the North Central Soybean Research Program and USDA-NIFA Hatch grant SD00H662-18.DetailsFiguresLiterature CitedRelated Vol. 104, No. 6 June 2020SubscribeISSN:0191-2917e-ISSN:1943-7692 DownloadCaptionSymptoms of citrus yellow mottle-associated virus on a leaf of Washington navel orange (J. X. Wu et al.). Photo credit: M. J. Cao. Effect of pydiflumetofen + difenoconazole on the severity of Cercospora leaf spot caused by Cercospora beticola in a small plot (S. J. Pethybridge et al.). Photo credit: S. J. Pethybridge. Metrics Article History Issue Date: 8 Jun 2020Published: 25 Mar 2020First Look: 24 Jan 2020Accepted: 20 Jan 2020 Pages: 1877-1877 Information© 2020 The American Phytopathological SocietyFundingNorth Central Soybean Research ProgramUSDA-NIFAGrant/Award Number: Hatch grant SD00H662-18Keywordsfungifield cropsoilseeds and legumespathogen detectionThe author(s) declare no conflict of interest.Cited byVirulence, Aggressiveness, and Fungicide Sensitivity of Phytophthora spp. Associated with Soybean in IllinoisDaniel Guillermo Cerritos Garcia, Shun-Yuan Huang, Nathan Michael Kleczewski, and Santiago Mideros22 November 2022 | Plant Disease, Vol. 0, No. jaPhytophthora sansomeana, an Emerging Threat to Soybean Production28 July 2022 | Agronomy, Vol. 12, No. 8Molecular mapping of quantitative disease resistance loci for soybean partial resistance to Phytophthora sansomeana15 March 2021 | Theoretical and Applied Genetics, Vol. 134, No. 7
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